PDGFC sirna

PDGFC siRNA (Rat)

Cat. Num. AAA8240105

• Gene Names: Pdgfc; SCDGF
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PDGFC; PDGFC siRNA (Rat); SCDGF; Platelet-derived growth factor C; PDGF-C; Fallotein; Spinal cord-derived growth factor; rScdfg; VEGF-E; PDGFC sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: PDGFC siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 345

• Applicable Applications for PDGFC sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat PDGFC gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PDGFC sirna

siRNA to inhibit PDGFC expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13786136
• NCBI GeneID: 79429
• NCBI Accession #: NP_112607.1
• NCBI GenBank Nucleotide #: NM_031317.1
• UniProt Accession #: Q9EQX6; Q8K429
• Molecular Weight: 38,734 Da
• NCBI Official Full Name: platelet-derived growth factor C
• NCBI Official Synonym Full Names: platelet derived growth factor C
• NCBI Official Symbol: Pdgfc
• NCBI Official Synonym Symbols: SCDGF
• NCBI Protein Information: platelet-derived growth factor C
• UniProt Protein Name: Platelet-derived growth factor C
• Protein Family: Platelet-derived growth factor
• UniProt Gene Name: Pdgfc
• UniProt Synonym Gene Names: Scdgf; PDGF-C; rScdfg; PDGFC latent form; PDGFC receptor-binding form
• UniProt Entry Name: PDGFC_RAT

NCBI Description

expressed in the embryonic spinal cord floor plate and ventricular zone, adult expression is localized to motoneurons in several regions of the brain [RGD, Feb 2006]

Uniprot Description

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen and chemoattractant for cells of mesenchymal origin. Required for normal skeleton formation during embryonic development, especially for normal development of the craniofacial skeleton and for normal development of the palate. Required for normal skin morphogenesis during embryonic development. Plays an important role in wound healing, where it appears to be involved in three stages: inflammation, proliferation and remodeling. Plays an important role in angiogenesis and blood vessel development. Involved in fibrotic processes, in which transformation of interstitial fibroblasts into myofibroblasts plus collagen deposition occurs. The CUB domain has mitogenic activity in coronary artery smooth muscle cells, suggesting a role beyond the maintenance of the latency of the PDGF domain. In the nucleus, PDGFC seems to have additional function ().