PDE4B sirna

PDE4B siRNA (Rat)

Cat. Num. AAA8208069

• Gene Names: Pde4b; Pde4B1; Pde4B2; Pde4B3; Pde4B4; PDE4/IVb
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PDE4B; PDE4B siRNA (Rat); cAMP-specific 3'.5'-cyclic phosphodiesterase 4B; DPDE4; PDE4B sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: PDE4B siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 721

• Applicable Applications for PDE4B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat PDE4B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PDE4B sirna

siRNA to inhibit PDE4B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 19923680
• NCBI GeneID: 24626
• NCBI Accession #: NP_058727.2
• NCBI GenBank Nucleotide #: NM_017031.2
• UniProt Accession #: P14646; Q5RKL0; Q8VD81; Q8VD82
• Molecular Weight: 81,737 Da
• NCBI Official Full Name: cAMP-specific 3',5'-cyclic phosphodiesterase 4B
• NCBI Official Synonym Full Names: phosphodiesterase 4B, cAMP specific
• NCBI Official Symbol: Pde4b
• NCBI Official Synonym Symbols: Pde4B1; Pde4B2; Pde4B3; Pde4B4; PDE4/IVb
• NCBI Protein Information: cAMP-specific 3',5'-cyclic phosphodiesterase 4B
• UniProt Protein Name: cAMP-specific 3',5'-cyclic phosphodiesterase 4B
• Protein Family: cAMP-specific 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: Pde4b
• UniProt Entry Name: PDE4B_RAT

NCBI Description

contains four isoforms expressed from the same gene; isoform Pde4b4 has cAMP specific phosphdiesterase activity and is inhibited by rolipram [RGD, Feb 2006]

Uniprot Description

PDE4B: a cAMP-specific phosphodiesterase. May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents. Inhibited by rolipram. Three alternatively-spliced isoforms have been described.

Protein type: Phosphodiesterase; EC 3.1.4.53; Nucleotide Metabolism - purine

Cellular Component: perinuclear region of cytoplasm; cytoplasm; voltage-gated calcium channel complex; Z disc; cytosol; nucleus

Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; metal ion binding; cAMP binding; 3',5'-cyclic-nucleotide phosphodiesterase activity

Biological Process: neutrophil chemotaxis; positive regulation of interferon-gamma production; smooth muscle contraction; metabolic process; cAMP catabolic process; positive regulation of interleukin-2 production; signal transduction; neutrophil homeostasis; leukocyte migration; T cell receptor signaling pathway