PDE4A sirna

PDE4A siRNA (Rat)

Cat. Num. AAA828528

• Gene Names: Pde4a; PHOSA
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PDE4A; PDE4A siRNA (Rat); cAMP-specific 3'.5'-cyclic phosphodiesterase 4A; DPDE2; PDE4A sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: PDE4A siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 844

• Applicable Applications for PDE4A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat PDE4A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PDE4A sirna

siRNA to inhibit PDE4A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 158518444
• NCBI GeneID: 25638
• NCBI Accession #: NP_037233.3
• NCBI GenBank Nucleotide #: NM_013101.3
• UniProt Accession #: P54748; P14645; Q9EQR7
• Molecular Weight: 85,344 Da
• NCBI Official Full Name: cAMP-specific 3',5'-cyclic phosphodiesterase 4A
• NCBI Official Synonym Full Names: phosphodiesterase 4A, cAMP-specific
• NCBI Official Symbol: Pde4a
• NCBI Official Synonym Symbols: PHOSA
• NCBI Protein Information: cAMP-specific 3',5'-cyclic phosphodiesterase 4A
• UniProt Protein Name: cAMP-specific 3',5'-cyclic phosphodiesterase 4A
• Protein Family: cAMP-specific 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: Pde4a
• UniProt Entry Name: PDE4A_RAT

NCBI Description

human homolog catalyzes the hydrolysis of cAMP; activity is inhibited by rolipram [RGD, Feb 2006]

Uniprot Description

PDE4A: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. Isoform 1 interacts with LYN. Isoform 2 and isoform 6 interact weakly with LYN. Isoform 1, isoform 2 and isoform 6 interact with ARRB2. Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum. Isoform 1, isoform 2, isoform 6 and isoform 7 are inhibited by rolipram and cilomilast. Isoform 1, isoform 2 and isoform 6 are inhibited by 4-[(3-butoxy-4-methoxyphenyl)-methyl]- 2-imidazolidinone (Ro 20-1724), roflumilast and denbufylline. Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.4.53; Nucleotide Metabolism - purine; Phosphodiesterase

Cellular Component: perinuclear region of cytoplasm; cytoplasm; nucleus; cytosol

Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; protein binding; metal ion binding; cAMP binding; 3',5'-cyclic-nucleotide phosphodiesterase activity

Biological Process: response to drug; metabolic process; sensory perception of smell; cAMP catabolic process; signal transduction