P2RY1 sirna

P2RY1 siRNA (Rat)

Cat. Num. AAA8234186

• Gene Names: P2ry1; P2y; P2y1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: P2RY1; P2RY1 siRNA (Rat); P2Y purinoceptor 1; P2Y1; ATP receptor; Purinergic receptor; P2RY1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: P2RY1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 373

• Applicable Applications for P2RY1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat P2RY1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for P2RY1 sirna

siRNA to inhibit P2RY1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 25742733
• NCBI GeneID: 25265
• NCBI Accession #: NP_036932.1
• NCBI GenBank Nucleotide #: NM_012800.1
• UniProt Accession #: P49651
• Molecular Weight: 42,322 Da
• NCBI Official Full Name: P2Y purinoceptor 1
• NCBI Official Synonym Full Names: purinergic receptor P2Y, G-protein coupled, 1
• NCBI Official Symbol: P2ry1
• NCBI Official Synonym Symbols: P2y; P2y1
• NCBI Protein Information: P2Y purinoceptor 1
• UniProt Protein Name: P2Y purinoceptor 1
• Protein Family: P2Y purinoceptor
• UniProt Gene Name: P2ry1
• UniProt Synonym Gene Names: P2Y1
• UniProt Entry Name: P2RY1_RAT

NCBI Description

G protein-coupled receptor for extracellular ATP; plays a role in ischemic preconditioning (IPC) in heart [RGD, Feb 2006]

Uniprot Description

P2RY1: Receptor for extracellular adenine nucleotides such as ATP and ADP. In platelets binding to ADP leads to mobilization of intracellular calcium ions via activation of phospholipase C, a change in platelet shape, and probably to platelet aggregation. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR; Membrane protein, integral

Cellular Component: postsynaptic membrane; cell surface; mitochondrion; basolateral plasma membrane; dendrite; apical plasma membrane; plasma membrane; integral to membrane

Molecular Function: ATP-activated nucleotide receptor activity; purinergic nucleotide receptor activity, G-protein coupled; protein heterodimerization activity; A1 adenosine receptor binding; ADP binding; ATP binding; ADP-activated nucleotide receptor activity

Biological Process: platelet activation; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); wound healing; eating behavior; regulation of vasodilation; positive regulation of hormone secretion; positive regulation of inositol trisphosphate biosynthetic process; sensory perception of pain; glial cell migration; negative regulation of binding; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; response to mechanical stimulus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of ion transport; positive regulation of protein amino acid phosphorylation; regulation of receptor activity; G-protein signaling, adenylate cyclase inhibiting pathway; adenosine receptor signaling pathway; aging