OTOF sirna

OTOF siRNA (Rat)

Cat. Num. AAA8220139

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: OTOF; OTOF siRNA (Rat); FER1L2; Otoferlin; Fer-1-like protein 2; OTOF sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: OTOF siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1993

• Applicable Applications for OTOF sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat OTOF gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for OTOF sirna

siRNA to inhibit OTOF expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 564355192
• NCBI GeneID: 84573
• NCBI Accession #: XP_006239895.1
• NCBI GenBank Nucleotide #: XM_006239833.2
• UniProt Accession #: Q9ERC5
• Molecular Weight: 226,339 Da
• NCBI Official Full Name: otoferlin isoform X3
• NCBI Official Synonym Full Names: otoferlin
• NCBI Official Symbol: Otof
• NCBI Protein Information: otoferlin
• UniProt Protein Name: Otoferlin
• Protein Family: Otoferlin
• UniProt Gene Name: Otof
• UniProt Synonym Gene Names: Fer1l2
• UniProt Entry Name: OTOF_RAT

NCBI Description

human homolog is associated with an autosomal recessive form of prelingual sensorineural deafness, DFNB9 [RGD, Feb 2006]

Uniprot Description

OTOF: Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion and in the control of neurotransmitter release at these output synapses. Interacts in a calcium-dependent manner to the presynaptic SNARE proteins at ribbon synapses of cochlear inner hair cells (IHCs) to trigger exocytosis of neurotransmitter. Also essential to synaptic exocytosis in immature outer hair cells (OHCs). May also play a role within the recycling of endosomes. Defects in OTOF are the cause of deafness autosomal recessive type 9 (DFNB9). DFNB9 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Defects in OTOF are a cause of auditory neuropathy, autosomal recessive, type 1 (AUNB1). A form of sensorineural hearing loss with absent or severely abnormal auditory brainstem response, in the presence of normal cochlear outer hair cell function and normal otoacoustic emissions. Auditory neuropathies result from a lesion in the area including the inner hair cells, connections between the inner hair cells and the cochlear branch of the auditory nerve, the auditory nerve itself and auditory pathways of the brainstem. In some cases AUNB1 phenotype can be temperature sensitive. Belongs to the ferlin family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Membrane protein, integral

Cellular Component: endoplasmic reticulum membrane; synaptic vesicle membrane; apical part of cell; basolateral plasma membrane; endoplasmic reticulum; cell; cytoplasm; integral to membrane; plasma membrane; cell junction

Molecular Function: protein binding; protein complex binding; calcium ion binding

Biological Process: synaptic vesicle exocytosis; sensory perception of sound