NPR1 sirna

NPR1 siRNA (Rat)

Cat. Num. AAA8217804

• Gene Names: Npr1; Gca; Npra; Anpra; NPR-A
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NPR1; NPR1 siRNA (Rat); Atrial natriuretic peptide receptor 1; Atrial natriuretic peptide receptor type A; ANP-A; ANPR-A; NPR-A; Guanylate cyclase A; GC-A; NPR1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: NPR1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,057

• Applicable Applications for NPR1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat NPR1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NPR1 sirna

siRNA to inhibit NPR1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6981280
• NCBI GeneID: 24603
• NCBI Accession #: NP_036745.1
• NCBI GenBank Nucleotide #: NM_012613.1
• UniProt Accession #: P18910; A1A5N8
• Molecular Weight: 118,951 Da
• NCBI Official Full Name: atrial natriuretic peptide receptor 1
• NCBI Official Synonym Full Names: natriuretic peptide receptor 1
• NCBI Official Symbol: Npr1
• NCBI Official Synonym Symbols: Gca; Npra; Anpra; NPR-A
• NCBI Protein Information: atrial natriuretic peptide receptor 1
• UniProt Protein Name: Atrial natriuretic peptide receptor 1
• Protein Family: Nitrogen permease reactivator protein
• UniProt Gene Name: Npr1
• UniProt Synonym Gene Names: ANP-A; ANPR-A; NPR-A; GC-A
• UniProt Entry Name: ANPRA_RAT

NCBI Description

receptor for atrial natriuretic peptide [RGD, Feb 2006]

Uniprot Description

ANPA: natriuretic peptide receptor A, which mediates natriuretic, diuretic, and vasorelaxing actions of the natriuretic peptides. Contains five functional domains: an extracellular ligand-binding domain, a single membrane-spanning region, and intracellularly a protein kinase homology domain, a helical hinge region involved in oligomerization, and a carboxyl-terminal guanylyl cyclase catalytic domain. Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Has guanylate cyclase activity on binding of ANF. Hypertensive SHR Rat has polymorphism at the locus. Human non-coding polymorphisms can alter expression up to two-fold, and have been associated with hypertension. A coding SNP is associated with hypertension and myocardial infarction.

Protein type: EC 4.6.1.2; Receptor, misc.; Protein kinase, RGC; Membrane protein, integral; Protein kinase, dual-specificity (receptor); Nucleotide Metabolism - purine; Guanylyl cyclase; Lyase; Kinase, protein; RGC group; RGC family

Cellular Component: plasma membrane; integral to membrane; receptor complex

Molecular Function: protein binding; guanylate cyclase activity; hormone binding; GTP binding; natriuretic peptide receptor activity; peptide hormone binding; protein kinase binding; ATP binding; protein kinase activity

Biological Process: cGMP biosynthetic process; cell surface receptor linked signal transduction; positive regulation of cGMP biosynthetic process; negative regulation of smooth muscle cell proliferation; regulation of blood pressure; receptor guanylyl cyclase signaling pathway; regulation of blood vessel size; protein amino acid phosphorylation; dopamine metabolic process