MYLK2 sirna

MYLK2 siRNA (Rat)

Cat. Num. AAA8202039

• Gene Names: Mylk2; Klmc; Mlck
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MYLK2; MYLK2 siRNA (Rat); Myosin light chain kinase 2. skeletal/cardiac muscle; MLCK2; MYLK2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: MYLK2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 610

• Applicable Applications for MYLK2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat MYLK2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MYLK2 sirna

siRNA to inhibit MYLK2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 17105364
• NCBI GeneID: 117558
• NCBI Accession #: NP_476557.1
• NCBI GenBank Nucleotide #: NM_057209.1
• UniProt Accession #: P20689
• Molecular Weight: 65,816 Da
• NCBI Official Full Name: myosin light chain kinase 2, skeletal/cardiac muscle
• NCBI Official Synonym Full Names: myosin light chain kinase 2
• NCBI Official Symbol: Mylk2
• NCBI Official Synonym Symbols: Klmc; Mlck
• NCBI Protein Information: myosin light chain kinase 2, skeletal/cardiac muscle
• UniProt Protein Name: Myosin light chain kinase 2, skeletal/cardiac muscle
• Protein Family: Myosin light chain kinase
• UniProt Gene Name: Mylk2
• UniProt Synonym Gene Names: MLCK2
• UniProt Entry Name: MYLK2_RAT

NCBI Description

kinase; phosphorylates a serine in the N-terminus of a myosin light chain [RGD, Feb 2006]

Uniprot Description

skMLCK: a calcium/calmodulin dependent kinase of the MLCK family. A myosin light chain kinase that is exclusively expressed in adult skeletal muscle.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.18; Motility/polarity/chemotaxis; Protein kinase, CAMK; CAMK group; MLCK family

Cellular Component: synaptic vesicle; cell soma; dendrite; postsynaptic density; cytoplasm; dendritic spine; terminal button; nucleus

Molecular Function: calmodulin binding; protein serine/threonine kinase activity; myosin light chain binding; ATP binding; myosin light chain kinase activity

Biological Process: striated muscle contraction; regulation of cell cycle; positive regulation of fast-twitch skeletal muscle contraction; regulation of muscle contraction; water transport; protein amino acid autophosphorylation; regulation of muscle filament sliding; peptidyl-threonine phosphorylation; synaptic vesicle transport; satellite cell differentiation; positive regulation of cell motility; protein amino acid phosphorylation; peptidyl-serine phosphorylation; muscle contraction; regulation of MAPKKK cascade; cardiac muscle morphogensis; neuromuscular synaptic transmission; regulation of neuronal synaptic plasticity