MC3R sirna

MC3R siRNA (Rat)

Cat. Num. AAA8205511

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MC3R; MC3R siRNA (Rat); Melanocortin receptor 3; MC3-R; MC3R sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: MC3R siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 323

• Applicable Applications for MC3R sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat MC3R gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MC3R sirna

siRNA to inhibit MC3R expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 148356234
• NCBI GeneID: 29310
• NCBI Accession #: NP_001020441.3
• NCBI GenBank Nucleotide #: NM_001025270.3
• UniProt Accession #: P32244; Q4KXA4
• Molecular Weight: 35,757 Da
• NCBI Official Full Name: melanocortin receptor 3
• NCBI Official Synonym Full Names: melanocortin 3 receptor
• NCBI Official Symbol: Mc3r
• NCBI Protein Information: melanocortin receptor 3
• UniProt Protein Name: Melanocortin receptor 3
• Protein Family: Melanocortin receptor
• UniProt Gene Name: Mc3r
• UniProt Synonym Gene Names: MC3-R
• UniProt Entry Name: MC3R_RAT

NCBI Description

receptor for corticotropin (ACTH) and melanotropin (MSH) [RGD, Feb 2006]

Uniprot Description

MC3R: Receptor for MSH (alpha, beta and gamma) and ACTH. This receptor is mediated by G proteins which activate adenylate cyclase. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR

Cellular Component: integral to plasma membrane

Molecular Function: neuropeptide binding; melanocortin receptor activity; melanocyte stimulating hormone receptor activity; peptide hormone binding

Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein coupled receptor protein signaling pathway; locomotor rhythm; regulation of heart rate; homoiothermy; regulation of blood pressure; positive regulation of cAMP biosynthetic process; sodium ion homeostasis; circadian regulation of gene expression