KHDRBS1 sirna

KHDRBS1 siRNA (Rat)

Cat. Num. AAA8224979

• Gene Names: Khdrbs1; P62; Sam68
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KHDRBS1; KHDRBS1 siRNA (Rat); SAM68; KH domain-containing. RNA-binding. signal transduction-associated protein 1; GAP-associated tyrosine phosphoprotein p62; Src-associated in mitosis 68 kDa protein; Sam68; p21 Ras GTPase-activating protein-associated p62; p68; KHDRBS1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: KHDRBS1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 443

• Applicable Applications for KHDRBS1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat KHDRBS1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KHDRBS1 sirna

siRNA to inhibit KHDRBS1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 18426824
• NCBI GeneID: 117268
• NCBI Accession #: NP_569089.1
• NCBI GenBank Nucleotide #: NM_130405.1
• UniProt Accession #: Q91V33
• Molecular Weight: 48,315 Da
• NCBI Official Full Name: KH domain-containing, RNA-binding, signal transduction-associated protein 1
• NCBI Official Synonym Full Names: KH domain containing, RNA binding, signal transduction associated 1
• NCBI Official Symbol: Khdrbs1
• NCBI Official Synonym Symbols: P62; Sam68
• NCBI Protein Information: KH domain-containing, RNA-binding, signal transduction-associated protein 1
• UniProt Protein Name: KH domain-containing, RNA-binding, signal transduction-associated protein 1
• Protein Family: KH domain-containing, RNA-binding, signal transduction-associated protein
• UniProt Gene Name: Khdrbs1
• UniProt Synonym Gene Names: Sam68
• UniProt Entry Name: KHDR1_RAT

NCBI Description

RNA binding protein and acts as a substrate of the Insulin receprot and may have a role as a docking protein in Insulin receptor signaling [RGD, Feb 2006]

Uniprot Description

KHDRBS1: a nuclear RNA-binding protein. May be involved in slicing and mRNA transport. Associates with src in mitosis. Binds to RNA via a heteronuclear ribonucleoprotein K homology domain. Exerts functionally separable effects on cell proliferation and survival, dependent on its ability to bind specifically to RNA.

Protein type: RNA-binding

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; SH3/SH2 adaptor activity; RNA binding; protein complex binding; poly(U) binding; SH3 domain binding; poly(A) binding

Biological Process: positive regulation of translational initiation; cell surface receptor linked signal transduction; transcription, DNA-dependent; positive regulation of signal transduction; positive regulation of RNA export from nucleus; regulation of signal transduction; regulation of protein stability; regulation of RNA export from nucleus; negative regulation of transcription, DNA-dependent; G2/M transition of mitotic cell cycle