KCNN2 sirna

KCNN2 siRNA (Rat)

Cat. Num. AAA8229920

• Gene Names: Kcnn2; SK2; SKCa2; KCa2.2; SKCa 2
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KCNN2; KCNN2 siRNA (Rat); Small conductance calcium-activated potassium channel protein 2; SK2; SKCa 2; SKCa2; KCa2.2; KCNN2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: KCNN2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 580

• Applicable Applications for KCNN2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat KCNN2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KCNN2 sirna

siRNA to inhibit KCNN2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 9506833
• NCBI GeneID: 54262
• NCBI Accession #: NP_062187.1
• NCBI GenBank Nucleotide #: NM_019314.2
• UniProt Accession #: P70604
• Molecular Weight: 63,848 Da
• NCBI Official Full Name: small conductance calcium-activated potassium channel protein 2 isoform 2
• NCBI Official Synonym Full Names: potassium channel, calcium activated intermediate/small conductance subfamily N alpha, member 2
• NCBI Official Symbol: Kcnn2
• NCBI Official Synonym Symbols: SK2; SKCa2; KCa2.2; SKCa 2
• NCBI Protein Information: small conductance calcium-activated potassium channel protein 2
• UniProt Protein Name: Small conductance calcium-activated potassium channel protein 2
• Protein Family: Small conductance calcium-activated potassium channel protein
• UniProt Gene Name: Kcnn2
• UniProt Synonym Gene Names: SK2; SKCa 2; SKCa2
• UniProt Entry Name: KCNN2_RAT

NCBI Description

small-conductance, calcium-activated potassium channel [RGD, Feb 2006]

Uniprot Description

KCNN2: Forms a voltage-independent potassium channel activated by intracellular calcium. Activation is followed by membrane hyperpolarization. Thought to regulate neuronal excitability by contributing to the slow component of synaptic afterhyperpolarization. The channel is blocked by apamin. Belongs to the potassium channel KCNN family. KCa2.2/KCNN2 subfamily.

Protein type: Channel, potassium; Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: smooth endoplasmic reticulum; cell soma; T-tubule; dendritic spine; plasma membrane; integral to membrane; Z disc

Molecular Function: calmodulin binding; calcium-activated potassium channel activity; protein binding; protein homodimerization activity; ion channel activity; alpha-actinin binding; small conductance calcium-activated potassium channel activity

Biological Process: ion transport; potassium ion transport; regulation of neuronal synaptic plasticity