KAT6A sirna

KAT6A siRNA (Rat)

Cat. Num. AAA827356

• Gene Names: Kat6a; Moz; Myst3; Znf220; Runxbp2
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KAT6A; KAT6A siRNA (Rat); MOZ; MYST3; Histone acetyltransferase KAT6A; MOZ. YBF2/SAS3. SAS2 and TIP60 protein 3; MYST-3; Monocytic leukemia zinc finger homolog; Monocytic leukemia zinc finger protein; KAT6A sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: KAT6A siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1998

• Applicable Applications for KAT6A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat KAT6A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KAT6A sirna

siRNA to inhibit KAT6A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 213972547
• NCBI GeneID: 306571
• NCBI Accession #: NP_001094040.1
• NCBI GenBank Nucleotide #: NM_001100570.2
• UniProt Accession #: Q5TKR9
• Molecular Weight: 223,331 Da
• NCBI Official Full Name: histone acetyltransferase KAT6A
• NCBI Official Synonym Full Names: K(lysine) acetyltransferase 6A
• NCBI Official Symbol: Kat6a
• NCBI Official Synonym Symbols: Moz; Myst3; Znf220; Runxbp2
• NCBI Protein Information: histone acetyltransferase KAT6A
• UniProt Protein Name: Histone acetyltransferase KAT6A
• Protein Family: Histone acetyltransferase
• UniProt Gene Name: Kat6a
• UniProt Synonym Gene Names: Moz; Myst3; MYST-3
• UniProt Entry Name: KAT6A_RAT

Uniprot Description

MYST3: Histone acetyltransferase that acetylates lysine residues in histone H3 and histone H4 (in vitro). Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. May act as a transcriptional coactivator for RUNX1 and RUNX2. Chromosomal aberrations involving KAT6A may be a cause of acute myeloid leukemias. Translocation t(8;16)(p11;p13) with CREBBP; translocation t(8;22)(p11;q13) with EP300. KAT6A- CREBBP may induce leukemia by inhibiting RUNX1-mediated transcription. Inversion inv(8)(p11;q13) generates the KAT6A-NCOA2 oncogene, which consists of the N-terminal part of KAT6A and the C-terminal part of NCOA2/TIF2. KAT6A-NCOA2 binds to CREBBP and disrupts its function in transcription activation. A chromosomal aberration involving KAT6A is a cause of therapy-related myelodysplastic syndrome. Translocation t(2;8)(p23;p11.2) with ASXL2 generates a KAT6A-ASXL2 fusion protein. Belongs to the MYST (SAS/MOZ) family.

Protein type: Oncoprotein; EC 2.3.1.48; Acetyltransferase; Nucleolus

Cellular Component: nucleoplasm; Golgi apparatus; PML body; nucleolus; nucleosome; nucleus

Molecular Function: protein binding; histone acetyltransferase activity; DNA binding; zinc ion binding; acetyltransferase activity; chromatin binding; transferase activity, transferring groups other than amino-acyl groups; transcription factor binding

Biological Process: heart morphogenesis; nucleosome assembly; protein amino acid acetylation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; embryonic hemopoiesis; somatic stem cell maintenance; metabolic process; positive regulation of transcription, DNA-dependent; negative regulation of transcription factor activity; myeloid cell differentiation; histone acetylation; negative regulation of transcription, DNA-dependent