INPPL1 sirna

INPPL1 siRNA (Rat)

Cat. Num. AAA8237654

• Gene Names: Inppl1; Ship2
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: INPPL1; INPPL1 siRNA (Rat); SHIP2; Phosphatidylinositol 3.4.5-trisphosphate 5-phosphatase 2; Inositol polyphosphate phosphatase-like protein 1; INPPL-1; SH2 domain-containing inositol 5'-phosphatase 2; SH2 domain-containing inositol phosphatase 2; SHIP-2; INPPL1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: INPPL1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,183

• Applicable Applications for INPPL1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat INPPL1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for INPPL1 sirna

siRNA to inhibit INPPL1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 399220355
• NCBI GeneID: 65038
• NCBI Accession #: NP_001257772.1
• NCBI GenBank Nucleotide #: NM_001270843.1
• UniProt Accession #: Q9WVR3; Q9R1V2
• Molecular Weight: 131,114 Da
• NCBI Official Full Name: phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2 isoform 2
• NCBI Official Synonym Full Names: inositol polyphosphate phosphatase-like 1
• NCBI Official Symbol: Inppl1
• NCBI Official Synonym Symbols: Ship2
• NCBI Protein Information: phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2
• UniProt Protein Name: Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2
• Protein Family: Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase
• UniProt Gene Name: Inppl1
• UniProt Synonym Gene Names: Ship2; INPPL-1; SH2 domain-containing inositol phosphatase 2; SHIP-2
• UniProt Entry Name: SHIP2_RAT

NCBI Description

phosphatase that plays a negative regulatory role in insulin-induced mitogenesis [RGD, Feb 2006]

Uniprot Description

SHIP-2: an SH2-containing inositol phosphatase. Recruited to activated receptor complexes including insulin R, PDGFR and Fc-gamma-R.

Protein type: EC 3.1.3.86; Phosphatase, lipid; Carbohydrate Metabolism - inositol phosphate; Motility/polarity/chemotaxis

Cellular Component: Golgi apparatus; cytoskeleton; membrane; lamellipodium; cytoplasm; plasma membrane; cytosol; filopodium

Molecular Function: protein binding; phosphatidylinositol-3,4,5-triphosphate binding; inositol-polyphosphate 5-phosphatase activity; SH2 domain binding; actin binding; SH3 domain binding

Biological Process: response to drug; negative regulation of MAP kinase activity; immune system process; negative regulation of insulin-like growth factor receptor signaling pathway; negative regulation of insulin receptor signaling pathway; glucose metabolic process; actin filament organization; endocytosis; cellular lipid metabolic process; inositol trisphosphate metabolic process; response to insulin stimulus; post-embryonic development; negative regulation of cell proliferation; negative regulation of DNA replication; phosphatidylinositol biosynthetic process; phosphoinositide dephosphorylation; brain development; cell adhesion; endochondral ossification