IMPAD1 sirna

IMPAD1 siRNA (Rat)

Cat. Num. AAA8224738

• Gene Names: Impad1; IMP 3; gPAPP; RGD1306455
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: IMPAD1; IMPAD1 siRNA (Rat); IMPA3; Inositol monophosphatase 3; IMP 3; IMPase 3; Golgi 3-prime phosphoadenosine 5-prime phosphate 3-prime phosphatase; Golgi-resident PAP phosphatase; gPAPP; Inositol monophosphatase domain-containing protein 1; Inositol-1(or 4)-monophosphatase 3; Myo-inositol monophosphatase A3; IMPAD1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: IMPAD1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 356

• Applicable Applications for IMPAD1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat IMPAD1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for IMPAD1 sirna

siRNA to inhibit IMPAD1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 291049776
• NCBI GeneID: 312952
• NCBI Accession #: NP_001008772.2
• NCBI GenBank Nucleotide #: NM_001008772.2
• UniProt Accession #: D4AD37
• Molecular Weight: 38,569 Da
• NCBI Official Full Name: inositol monophosphatase 3
• NCBI Official Synonym Full Names: inositol monophosphatase domain containing 1
• NCBI Official Symbol: Impad1
• NCBI Official Synonym Symbols: IMP 3; gPAPP; RGD1306455
• NCBI Protein Information: inositol monophosphatase 3
• UniProt Protein Name: Inositol monophosphatase 3
• Protein Family: Inositol monophosphatase
• UniProt Gene Name: Impad1
• UniProt Synonym Gene Names: Impa3; IMP 3; IMPase 3; Golgi-resident PAP phosphatase; gPAPP
• UniProt Entry Name: IMPA3_RAT

Uniprot Description

IMPAD1: May play a role in the formation of skeletal elements derived through endochondral ossification, possibly by clearing adenosine 3',5'-bisphosphate produced by Golgi sulfotransferases during glycosaminoglycan sulfation. Defects in IMPAD1 are the cause of chondrodysplasia with joint dislocations GPAPP type (CDP-GPAPP). A condition consisting of congenital joint dislocations, chondrodysplasia with short stature, micrognathia and cleft palate, and a distinctive face. Belongs to the inositol monophosphatase family.

Protein type: Hydrolase; Phosphatase (non-protein); Membrane protein, integral; Cell development/differentiation; EC 3.1.3.7; EC 3.1.3.25

Cellular Component: Golgi apparatus; membrane; integral to membrane

Molecular Function: 3'-nucleotidase activity; metal ion binding; inositol-1(or 4)-monophosphatase activity; 3'(2'),5'-bisphosphate nucleotidase activity

Biological Process: chondroitin sulfate metabolic process; dephosphorylation; phosphoinositide phosphorylation; inositol biosynthetic process; embryonic digit morphogenesis; chondrocyte development; skeletal development; post-embryonic development; endochondral ossification