IKBKB sirna

IKBKB siRNA (Rat)

Cat. Num. AAA8238459

• Gene Names: Ikbkb; IKK2; AIM-1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: IKBKB; IKBKB siRNA (Rat); IKKB; Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta; NFKBIKB; IKBKB sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: IKBKB siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 757

• Applicable Applications for IKBKB sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat IKBKB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for IKBKB sirna

siRNA to inhibit IKBKB expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 158508716
• NCBI GeneID: 84351
• NCBI Accession #: NP_445807.2
• NCBI GenBank Nucleotide #: NM_053355.2
• UniProt Accession #: Q9QY78
• Molecular Weight: 86,866 Da
• NCBI Official Full Name: inhibitor of nuclear factor kappa-B kinase subunit beta
• NCBI Official Synonym Full Names: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
• NCBI Official Symbol: Ikbkb
• NCBI Official Synonym Symbols: IKK2; AIM-1
• NCBI Protein Information: inhibitor of nuclear factor kappa-B kinase subunit beta
• UniProt Protein Name: Inhibitor of nuclear factor kappa-B kinase subunit beta
• UniProt Gene Name: Ikbkb
• UniProt Synonym Gene Names: Ikkb; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; IKK2; NFKBIKB
• UniProt Entry Name: IKKB_RAT

NCBI Description

kinase that may be involved in megakaryocyte polyploidization [RGD, Feb 2006]

Uniprot Description

IKKB: a kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Preferentially found as a heterodimer with IKK-alpha but also as an homodimer.

Protein type: Kinase, protein; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.10; Other group; IKK family

Cellular Component: internal side of plasma membrane; intracellular membrane-bound organelle; cytoplasm; IkappaB kinase complex; cytosol; nucleus; lipid raft

Molecular Function: protein binding; protein homodimerization activity; IkappaB kinase activity; protein heterodimerization activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: skeletal muscle contraction; response to drug; serine phosphorylation of STAT protein; B cell homeostasis; response to toxin; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; I-kappaB phosphorylation; protein amino acid phosphorylation; neurite development; activation of NF-kappaB transcription factor