IDO1 sirna

IDO1 siRNA (Rat)

Cat. Num. AAA8207121

• Gene Names: Ido1; Ido; Indo
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: IDO1; IDO1 siRNA (Rat); IDO; INDO; Indoleamine 2.3-dioxygenase 1; IDO-1; Indoleamine-pyrrole 2.3-dioxygenase; IDO1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: IDO1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 407

• Applicable Applications for IDO1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat IDO1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for IDO1 sirna

siRNA to inhibit IDO1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13027384
• NCBI GeneID: 66029
• NCBI Accession #: NP_076463.1
• NCBI GenBank Nucleotide #: NM_023973.1
• UniProt Accession #: Q9ERD9
• Molecular Weight: 45,831 Da
• NCBI Official Full Name: indoleamine 2,3-dioxygenase 1
• NCBI Official Synonym Full Names: indoleamine 2,3-dioxygenase 1
• NCBI Official Symbol: Ido1
• NCBI Official Synonym Symbols: Ido; Indo
• NCBI Protein Information: indoleamine 2,3-dioxygenase 1
• UniProt Protein Name: Indoleamine 2,3-dioxygenase 1
• Protein Family: Indoleamine 2,3-dioxygenase
• UniProt Gene Name: Ido1
• UniProt Synonym Gene Names: Ido; Indo; IDO-1
• UniProt Entry Name: I23O1_RAT

NCBI Description

enzyme which catalyzes degradation of L-tryptophan to N-formylkynurenine; human homolog is induced by gamma-interferon and is believed to be involved in that agent's tumor antiproliferative effects [RGD, Feb 2006]

Uniprot Description

INDO: Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. Belongs to the indoleamine 2,3-dioxygenase family.

Protein type: Apoptosis; Oxidoreductase; Cell cycle regulation; Contractile; Amino Acid Metabolism - tryptophan; EC 1.13.11.52

Cellular Component: stereocilium bundle; smooth muscle contractile fiber; cytoplasm; cytosol

Molecular Function: amino acid binding; metal ion binding; heme binding; indoleamine 2,3-dioxygenase activity; oxygen binding; tryptophan 2,3-dioxygenase activity

Biological Process: regulation of activated T cell proliferation; positive regulation of T-helper 2 type immune response; negative regulation of interleukin-10 production; positive regulation of chronic inflammatory response; positive regulation of apoptosis; positive regulation of interleukin-12 production; tryptophan catabolic process to kynurenine; negative regulation of activated T cell proliferation; response to lipopolysaccharide; negative regulation of T cell proliferation; cytokine production during acute inflammatory response; inflammatory response; multicellular organismal response to stress; positive regulation of T cell tolerance induction