HK3 sirna

HK3 siRNA (Rat)

Cat. Num. AAA8225945

• Gene Names: Hk3; RNU73859
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HK3; HK3 siRNA (Rat); Hexokinase-3; Hexokinase type III; HK III; HK3 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: HK3 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 924

• Applicable Applications for HK3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat HK3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HK3 sirna

siRNA to inhibit HK3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 11559937
• NCBI GeneID: 25060
• NCBI Accession #: NP_071515.1
• NCBI GenBank Nucleotide #: NM_022179.2
• UniProt Accession #: P27926; Q497A1
• Molecular Weight: 100,254 Da
• NCBI Official Full Name: hexokinase-3
• NCBI Official Synonym Full Names: hexokinase 3 (white cell)
• NCBI Official Symbol: Hk3
• NCBI Official Synonym Symbols: RNU73859
• NCBI Protein Information: hexokinase-3
• UniProt Protein Name: Hexokinase-3
• Protein Family: Probable histidine kinase
• UniProt Gene Name: Hk3
• UniProt Synonym Gene Names: HK III
• UniProt Entry Name: HXK3_RAT

NCBI Description

ATP:D-hexose 6-phosphotransferase type III isozyme; member of a family of hexokinase isozymes [RGD, Feb 2006]

Uniprot Description

HK3: a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). Acts as a ""glucose sensor"" by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 2.7.1.1; Kinase, other; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Enzyme, cellular metabolism; Transferase; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - starch and sucrose

Cellular Component: protein complex; mitochondrion; cytosol

Molecular Function: protein binding; hexokinase activity; enzyme binding; mannokinase activity; hormone binding; fructokinase activity; glucokinase activity; ATP binding

Biological Process: hexose metabolic process; glycolysis; glucose 6-phosphate metabolic process; carbohydrate phosphorylation; cell glucose homeostasis