HES1 sirna

HES1 siRNA (Rat)

Cat. Num. AAA8208190

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HES1; HES1 siRNA (Rat); HES-1; HL; Transcription factor HES-1; Hairy and enhancer of split 1; Hairy-like protein; RHL; HES1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: HES1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 281

• Applicable Applications for HES1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat HES1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HES1 sirna

siRNA to inhibit HES1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 148747225
• NCBI GeneID: 29577
• NCBI Accession #: NP_077336.3
• NCBI GenBank Nucleotide #: NM_024360.3
• UniProt Accession #: Q04666
• Molecular Weight: 29,622 Da
• NCBI Official Full Name: transcription factor HES-1
• NCBI Official Synonym Full Names: hes family bHLH transcription factor 1
• NCBI Official Symbol: Hes1
• NCBI Protein Information: transcription factor HES-1
• UniProt Protein Name: Transcription factor HES-1
• Protein Family: Protein
• UniProt Gene Name: Hes1
• UniProt Synonym Gene Names: Hes-1; Hl
• UniProt Entry Name: HES1_RAT

NCBI Description

may act as a transcriptional repressor; may inhibit cell adhesion molecule NCAM-dependent neurite outgrowth [RGD, Feb 2006]

Uniprot Description

HES1: Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity. May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage.

Protein type: Transcription factor; Cell development/differentiation; Motility/polarity/chemotaxis

Cellular Component: nucleoplasm; nuclear matrix; cytoplasm; intracellular; chromatin; nucleus

Molecular Function: JUN kinase binding; protein binding; protein homodimerization activity; DNA binding; sequence-specific DNA binding; chaperone binding; histone deacetylase binding; protein complex binding; chromatin binding; transcription factor binding; transcription factor activity

Biological Process: neural tube development; radial glial cell differentiation in the forebrain; hindbrain morphogenesis; somatic stem cell maintenance; regulation of fat cell differentiation; positive regulation of transcription, DNA-dependent; cell maturation; positive regulation of JAK-STAT cascade; positive regulation of glial cell differentiation; response to organic cyclic substance; positive regulation of tyrosine phosphorylation of Stat3 protein; neuron differentiation; midbrain development; protein complex assembly; positive regulation of T cell proliferation; positive regulation of mitotic cell cycle, embryonic; cell adhesion; positive regulation of astrocyte differentiation; positive regulation of DNA binding; cell fate commitment; transcription, DNA-dependent; auditory receptor cell fate determination; hair cell differentiation; regulation of transcription from RNA polymerase II promoter; negative regulation of neuron differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; transcription from RNA polymerase II promoter; response to alkaloid; midbrain-hindbrain boundary morphogenesis; regulation of protein heterodimerization activity; regulation of timing of neuron differentiation; auditory receptor cell differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of neurogenesis; regulation of neurogenesis; positive regulation of cell proliferation; pancreas development; adenohypophysis development; trochlear nerve development; oculomotor nerve development; regulation of timing of cell differentiation; positive regulation of Notch signaling pathway; positive regulation of BMP signaling pathway; STAT protein nuclear translocation; Notch signaling pathway; smoothened signaling pathway; cell migration; thymus development; in utero embryonic development; liver development; pattern specification process; negative regulation of oligodendrocyte differentiation; telencephalon development; negative regulation of cell differentiation; lateral inhibition; pituitary gland development; negative regulation of auditory receptor cell differentiation; neuron morphogenesis during differentiation; artery morphogenesis; lung development