GUCY2F sirna

GUCY2F siRNA (Rat)

Cat. Num. AAA8221936

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GUCY2F; GUCY2F siRNA (Rat); GUC2F; Retinal guanylyl cyclase 2; RETGC-2; Guanylate cyclase 2F. retinal; Guanylate cyclase F; GC-F; Rod outer segment membrane guanylate cyclase 2; ROS-GC2; GUCY2F sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: GUCY2F siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1108

• Applicable Applications for GUCY2F sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat GUCY2F gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GUCY2F sirna

siRNA to inhibit GUCY2F expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 16758684
• NCBI GeneID: 116556
• NCBI Accession #: NP_446283.1
• NCBI GenBank Nucleotide #: NM_053831.1
• UniProt Accession #: P51842
• Molecular Weight: 124,411 Da
• NCBI Official Full Name: retinal guanylyl cyclase 2
• NCBI Official Synonym Full Names: guanylate cyclase 2F
• NCBI Official Symbol: Gucy2f
• NCBI Protein Information: retinal guanylyl cyclase 2
• UniProt Protein Name: Retinal guanylyl cyclase 2
• Protein Family: Retinal guanylyl cyclase
• UniProt Gene Name: Gucy2f
• UniProt Synonym Gene Names: Guc2f; RETGC-2; GC-F; ROS-GC2
• UniProt Entry Name: GUC2F_RAT

NCBI Description

membrane guanylyl cyclase orphan receptor; may play functional role with the rods and/or cones of photoreceptor cells [RGD, Feb 2006]

Uniprot Description

GUCY2F: Probably plays a specific functional role in the rods and/or cones of photoreceptors. It may be the enzyme involved in the resynthesis of cGMP required for recovery of the dark state after phototransduction. Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.

Protein type: Nucleotide Metabolism - purine; Kinase, protein; Protein kinase, RGC; Protein kinase, dual-specificity (receptor); Membrane protein, integral; Receptor, misc.; EC 4.6.1.2; Lyase; Guanylyl cyclase; RGC group; RGC family

Cellular Component: membrane; integral to membrane

Molecular Function: protein binding; guanylate cyclase activity; protein homodimerization activity; GTP binding; protein heterodimerization activity; ATP binding; protein kinase activity

Biological Process: cGMP biosynthetic process; visual perception; protein amino acid phosphorylation