GSS sirna

GSS siRNA (Rat)

Cat. Num. AAA8223322

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GSS; GSS siRNA (Rat); Glutathione synthetase; GSH synthetase; GSH-S; Glutathione synthase; GSS sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: GSS siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 474

• Applicable Applications for GSS sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat GSS gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GSS sirna

siRNA to inhibit GSS expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 25742757
• NCBI GeneID: 25458
• NCBI Accession #: NP_037094.1
• NCBI GenBank Nucleotide #: NM_012962.1
• UniProt Accession #: P46413
• Molecular Weight: 52,345 Da
• NCBI Official Full Name: glutathione synthetase
• NCBI Official Synonym Full Names: glutathione synthetase
• NCBI Official Symbol: Gss
• NCBI Protein Information: glutathione synthetase
• UniProt Protein Name: Glutathione synthetase
• Protein Family: Glutathione synthetase
• UniProt Gene Name: Gss
• UniProt Synonym Gene Names: GSH synthetase; GSH-S
• UniProt Entry Name: GSHB_RAT

NCBI Description

catalyzes the synthesis of glutathione from gamma-L-glutamyl-L-cysteine and glycine; activity is ATP-dependent [RGD, Feb 2006]

Uniprot Description

GSS: Defects in GSS are the cause of glutathione synthetase deficiency (GSS deficiency); also known as 5- oxoprolinuria or pyroglutamic aciduria. It is a severe form characterized by an increased rate of hemolysis and defective function of the central nervous system. Defects in GSS are the cause of glutathione synthetase deficiency of erythrocytes (GLUSYNDE)[MIM:231900]. Glutathione synthetase deficiency of erythrocytes is a mild form causing hemolytic anemia. Belongs to the eukaryotic GSH synthase family.

Protein type: Ligase; EC 6.3.2.3; Other Amino Acids Metabolism - glutathione

Molecular Function: protein homodimerization activity; glutathione binding; glycine binding; magnesium ion binding; glutathione synthase activity; peptide binding; ATP binding

Biological Process: response to nutrient levels; response to xenobiotic stimulus; response to cadmium ion; glutathione biosynthetic process; response to amino acid stimulus; aging