GRIN1 sirna

GRIN1 siRNA (Rat)

Cat. Num. AAA8214189

• Gene Names: Grin1; NR1; GluN1; NMDAR1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GRIN1; GRIN1 siRNA (Rat); NMDAR1; Glutamate receptor ionotropic. NMDA 1; GluN1; Glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; NMD-R1; GRIN1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: GRIN1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 959

• Applicable Applications for GRIN1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat GRIN1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GRIN1 sirna

siRNA to inhibit GRIN1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 396578144
• NCBI GeneID: 24408
• NCBI Accession #: NP_001257531.1
• NCBI GenBank Nucleotide #: NM_001270602.1
• UniProt Accession #: P35439; Q62646
• Molecular Weight: 101,987 Da
• NCBI Official Full Name: glutamate receptor ionotropic, NMDA 1 isoform 1b
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, N-methyl D-aspartate 1
• NCBI Official Symbol: Grin1
• NCBI Official Synonym Symbols: NR1; GluN1; NMDAR1
• NCBI Protein Information: glutamate receptor ionotropic, NMDA 1
• UniProt Protein Name: Glutamate receptor ionotropic, NMDA 1
• Protein Family: Glutamate receptor ionotropic
• UniProt Gene Name: Grin1
• UniProt Synonym Gene Names: Nmdar1; GluN1; NMD-R1
• UniProt Entry Name: NMDZ1_RAT

NCBI Description

subunit of NMDA-preferring ionotropic glutamate receptors; may play a role in long term potentiation [RGD, Feb 2006]

Uniprot Description

NMDAR1: a subunit of N-methyl-D-aspartate (NMDA) receptors, members of the glutamate receptor channel superfamily. Possesses high calcium permeability and voltage-dependent sensitivity to magnesium and is modulated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission. Three alternatively-spliced isoforms have been described.

Protein type: Membrane protein, integral; Channel, ligand-gated; Channel, calcium; Membrane protein, multi-pass

Cellular Component: cell surface; integral to plasma membrane; cell; endoplasmic reticulum; dendrite; postsynaptic density; dendritic spine; terminal button; excitatory synapse; N-methyl-D-aspartate selective glutamate receptor complex; postsynaptic membrane; synaptic vesicle; membrane; cell soma; cytoplasm; synapse; cell junction

Molecular Function: voltage-gated cation channel activity; protein dimerization activity; neurotransmitter binding; glutamate receptor binding; calcium channel activity; calcium ion binding; calmodulin binding; protein binding; enzyme binding; glutamate binding; extracellular-glutamate-gated ion channel activity; ionotropic glutamate receptor activity; protein heterodimerization activity; glycine binding; N-methyl-D-aspartate selective glutamate receptor activity; cation channel activity; receptor binding

Biological Process: regulation of long-term neuronal synaptic plasticity; startle response; male mating behavior; prepulse inhibition; positive regulation of apoptosis; adult locomotory behavior; regulation of dendrite morphogenesis; rhythmic process; response to morphine; regulation of synaptic plasticity; regulation of axonogenesis; sensory perception of pain; response to organic cyclic substance; calcium ion homeostasis; regulation of respiratory gaseous exchange; learning and/or memory; conditioned taste aversion; calcium ion transport; regulation of neuron apoptosis; visual learning; negative regulation of neuron apoptosis; associative learning; cation transport; protein tetramerization; synaptic transmission, glutamatergic; neuromuscular process; response to amphetamine; social behavior; learning; respiratory gaseous exchange; pons maturation; memory; cellular calcium ion homeostasis; regulation of membrane potential; response to ethanol; suckling behavior; regulation of synaptogenesis; long-term memory; olfactory learning; propylene metabolic process; ionotropic glutamate receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; cerebral cortex development; response to calcium ion; regulation of excitatory postsynaptic membrane potential; regulation of neuronal synaptic plasticity; response to amine stimulus