GLRB sirna

GLRB siRNA (Rat)

Cat. Num. AAA8226070

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GLRB; GLRB siRNA (Rat); Glycine receptor subunit beta; Glycine receptor 58 kDa subunit; GLRB sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: GLRB siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 496

• Applicable Applications for GLRB sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat GLRB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GLRB sirna

siRNA to inhibit GLRB expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 17865325
• NCBI GeneID: 25456
• NCBI Accession #: NP_445748.1
• NCBI GenBank Nucleotide #: NM_053296.1
• UniProt Accession #: P20781
• Molecular Weight: 55,927 Da
• NCBI Official Full Name: glycine receptor subunit beta
• NCBI Official Synonym Full Names: glycine receptor, beta
• NCBI Official Symbol: Glrb
• NCBI Protein Information: glycine receptor subunit beta
• UniProt Protein Name: Glycine receptor subunit beta
• Protein Family: Glycine receptor
• UniProt Gene Name: Glrb
• UniProt Entry Name: GLRB_RAT

NCBI Description

plays a role in synaptic transmission [RGD, Feb 2006]

Uniprot Description

GLRB: The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing). Defects in GLRB are the cause of hyperekplexia type 2 (HKPX2). HKPX2 is a neurologic disorder characterized by muscular rigidity of central nervous system origin, particularly in the neonatal period, and by an exaggerated startle response to unexpected acoustic or tactile2 stimuli. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glycine receptor (TC 1.A.9.3) subfamily. GLRB sub- subfamily.

Protein type: Transporter, ion channel; Membrane protein, multi-pass; Membrane protein, integral; Transporter; Channel, ligand-gated

Cellular Component: postsynaptic membrane; membrane; cell; integral to membrane; cell junction

Molecular Function: protein binding; extracellular-glycine-gated chloride channel activity; glycine binding; extracellular-glycine-gated ion channel activity; drug binding

Biological Process: synaptic transmission; nervous system development; regulation of membrane potential; startle response; synaptic transmission, glycinergic; visual perception; righting reflex; neuropeptide signaling pathway; protein heterooligomerization; neuromuscular process; acrosome reaction; ion transport; adult walking behavior