GJC2 sirna

GJC2 siRNA (Rat)

Cat. Num. AAA8218980

• Gene Names: Gjc2; Cx47; Gja12
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GJC2; GJC2 siRNA (Rat); GJA12; Gap junction gamma-2 protein; Connexin-47; Cx47; Gap junction alpha-12 protein; GJC2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: GJC2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 440

• Applicable Applications for GJC2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat GJC2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GJC2 sirna

siRNA to inhibit GJC2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 294832037
• NCBI GeneID: 497913
• NCBI Accession #: NP_001094254.1
• NCBI GenBank Nucleotide #: NM_001100784.1
• UniProt Accession #: Q80XF7
• Molecular Weight: 46,990 Da
• NCBI Official Full Name: gap junction gamma-2 protein
• NCBI Official Synonym Full Names: gap junction protein, gamma 2
• NCBI Official Symbol: Gjc2
• NCBI Official Synonym Symbols: Cx47; Gja12
• NCBI Protein Information: gap junction gamma-2 protein
• UniProt Protein Name: Gap junction gamma-2 protein
• Protein Family: Gap junction gamma-2 protein
• UniProt Gene Name: Gjc2
• UniProt Synonym Gene Names: Gja12; Cx47
• UniProt Entry Name: CXG2_RAT

Uniprot Description

GJA12: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a role in myelination in central and peripheral nervous systems. Defects in GJC2 are the cause of leukodystrophy hypomyelinating type 2 (HLD2); also known as Pelizaeus-Merzbacher-like disease autosomal recessive type 1. HLD2 is an autosomal recessive hypomyelinating leukodystrophy characterized by nystagmus, impaired motor development, ataxia, choreoathetotic movements, dysarthria and progressive spasticity. Defects in GJC2 are the cause of spastic paraplegia autosomal recessive type 44 (SPG44). A form of spastic paraplegia, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. Defects in GJC2 are the cause of lymphedema hereditary type 1C (LMPH1C). LMPH1C is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections and physical impairment. Belongs to the connexin family. Gamma-type subfamily.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Cellular Component: connexon complex; integral to membrane; gap junction; myelin sheath

Molecular Function: gap junction channel activity

Biological Process: cell-cell signaling; response to toxin; transmembrane transport