GIPR sirna

GIPR siRNA (Rat)

Cat. Num. AAA8200381

• Gene Names: Gipr; Gippr; RATGIPPR
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GIPR; GIPR siRNA (Rat); Gastric inhibitory polypeptide receptor; GIP-R; Glucose-dependent insulinotropic polypeptide receptor; GIPR sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: GIPR siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 455

• Applicable Applications for GIPR sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat GIPR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GIPR sirna

siRNA to inhibit GIPR expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6978894
• NCBI GeneID: 25024
• NCBI Accession #: NP_036846.1
• NCBI GenBank Nucleotide #: NM_012714.1
• UniProt Accession #: P43219
• Molecular Weight: 52,257 Da
• NCBI Official Full Name: gastric inhibitory polypeptide receptor
• NCBI Official Synonym Full Names: gastric inhibitory polypeptide receptor
• NCBI Official Symbol: Gipr
• NCBI Official Synonym Symbols: Gippr; RATGIPPR
• NCBI Protein Information: gastric inhibitory polypeptide receptor
• UniProt Protein Name: Gastric inhibitory polypeptide receptor
• Protein Family: Gastric inhibitory polypeptide receptor
• UniProt Gene Name: Gipr
• UniProt Synonym Gene Names: GIP-R
• UniProt Entry Name: GIPR_RAT

NCBI Description

receptor for gastric inhibitory polypeptide/glucose-dependent insulinotropic peptide; induces cAMP production; mediates increase in intracellular calcium ions [RGD, Feb 2006]

Uniprot Description

GIPR: This is a receptor for GIP. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Belongs to the G-protein coupled receptor 2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GPCR, family 2; Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral

Cellular Component: integral to membrane; plasma membrane

Molecular Function: gastric inhibitory peptide receptor activity; peptide hormone binding

Biological Process: desensitization of G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; positive regulation of cAMP biosynthetic process; response to glucose stimulus; positive regulation of insulin secretion; response to axon injury; response to calcium ion; endocrine pancreas development