GDF6 sirna

GDF6 siRNA (Rat)

Cat. Num. AAA8228041

• Gene Names: Gdf6; gdf16; BMP-13
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GDF6; GDF6 siRNA (Rat); BMP13; GDF16; Growth/differentiation factor 6; GDF-6; Bone morphogenetic protein 13; BMP-13; Growth/differentiation factor 16; GDF6 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: GDF6 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 452

• Applicable Applications for GDF6 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat GDF6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GDF6 sirna

siRNA to inhibit GDF6 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 61556763
• NCBI GeneID: 252834
• NCBI Accession #: NP_001013056.1
• NCBI GenBank Nucleotide #: NM_001013038.1
• UniProt Accession #: Q6HA10; Q8K4X4
• Molecular Weight: 51,013 Da
• NCBI Official Full Name: growth/differentiation factor 6
• NCBI Official Synonym Full Names: growth differentiation factor 6
• NCBI Official Symbol: Gdf6
• NCBI Official Synonym Symbols: gdf16; BMP-13
• NCBI Protein Information: growth/differentiation factor 6
• UniProt Protein Name: Growth/differentiation factor 6
• Protein Family: Growth/differentiation factor
• UniProt Gene Name: Gdf6
• UniProt Synonym Gene Names: Bmp13; Gdf16; GDF-6; BMP-13
• UniProt Entry Name: GDF6_RAT

NCBI Description

member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily; regulates cell growth and differentiation; involved with tendon and ligament formation [RGD, Feb 2006]

Uniprot Description

Growth factor that controls proliferation and cellular differentiation in the retina and bone formation. Plays a key role in regulating apoptosis during retinal development. Establishes dorsal-ventral positional information in the retina and controls the formation of the retinotectal map. Required for normal formation of bones and joints in the limbs, skull, digits and axial skeleton. Plays a key role in establishing boundaries between skeletal elements during development. Regulation of GDF6 expression seems to be a mechanism for evolving species-specific changes in skeletal strucutres. Seems to positively regulates differentiation of chondrogenic tissue through the growth factor receptors subunits BMPR1A, BMPR1B, BMPR2 and ACVR2A, leading to the activation of SMAD1-SMAD5-SMAD8 complex. The regulation of chondrogenic differentiation is inhibited by NOG. Also involved in the induction of adipogenesis from mesenchymal stem cells. This mechanism acts through the growth factor receptors subunits BMPR1A, BMPR2 and ACVR2A and the activation of SMAD1-SMAD5-SMAD8 complex and MAPK14/p38.