EGR1 sirna

EGR1 siRNA (Rat)

Cat. Num. AAA8227270

• Gene Names: Egr1; Ngf1; Ngfi; NGFI-A; Krox-24; zif-268
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EGR1; EGR1 siRNA (Rat); Early growth response protein 1; EGR-1; Nerve growth factor-induced protein A; NGFI-A; Transcription factor Zif268; Zinc finger protein Krox-24; EGR1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: EGR1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 508

• Applicable Applications for EGR1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat EGR1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EGR1 sirna

siRNA to inhibit EGR1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6978799
• NCBI GeneID: 24330
• NCBI Accession #: NP_036683.1
• NCBI GenBank Nucleotide #: NM_012551.2
• UniProt Accession #: P08154; Q53YM5
• Molecular Weight: 53,935 Da
• NCBI Official Full Name: early growth response protein 1
• NCBI Official Synonym Full Names: early growth response 1
• NCBI Official Symbol: Egr1
• NCBI Official Synonym Symbols: Ngf1; Ngfi; NGFI-A; Krox-24; zif-268
• NCBI Protein Information: early growth response protein 1
• UniProt Protein Name: Early growth response protein 1
• Protein Family: Early growth response protein
• UniProt Gene Name: Egr1
• UniProt Synonym Gene Names: EGR-1; NGFI-A
• UniProt Entry Name: EGR1_RAT

NCBI Description

activates transcription of the LH receptor gene; may be involved in synaptic plasticity during REM sleep [RGD, Feb 2006]

Uniprot Description

EGR1: Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation. By growth factors. Belongs to the EGR C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein; DNA-binding; Transcription factor

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function: DNA binding; double-stranded DNA binding; histone acetyltransferase binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding

Biological Process: BMP signaling pathway; cellular response to insulin stimulus; circadian rhythm; learning and/or memory; long-term memory; negative regulation of transcription from RNA polymerase II promoter; oligodendrocyte differentiation; positive regulation of chemokine biosynthetic process; positive regulation of hormone biosynthetic process; positive regulation of interleukin-1 beta biosynthetic process; positive regulation of neuron apoptosis; positive regulation of smooth muscle cell migration; positive regulation of smooth muscle cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of apoptosis; regulation of long-term neuronal synaptic plasticity; regulation of protein sumoylation; regulation of transcription, DNA-dependent; response to amphetamine; response to cocaine; response to drug; response to electrical stimulus; response to ethanol; response to follicle-stimulating hormone stimulus; response to glucose stimulus; response to hypoxia; response to insulin stimulus; response to nutrient levels; T cell differentiation; transcription from RNA polymerase II promoter; wound healing