DYNLL1 sirna

DYNLL1 siRNA (Rat)

Cat. Num. AAA8237694

• Gene Names: Dynll1; Pin; Dlc8; 8kDLC; Dnclc1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DYNLL1; DYNLL1 siRNA (Rat); DNCL1; DNCLC1; PIN; Dynein light chain 1 cytoplasmic; 8 kDa dynein light chain; DLC8; Dynein light chain LC8-type 1; Protein inhibitor of neuronal nitric oxide synthase; DYNLL1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: DYNLL1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 89

• Applicable Applications for DYNLL1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat DYNLL1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DYNLL1 sirna

siRNA to inhibit DYNLL1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 16758016
• NCBI GeneID: 58945
• NCBI Accession #: NP_445771.1
• NCBI GenBank Nucleotide #: NM_053319.3
• UniProt Accession #: P63170; Q15701
• Molecular Weight: 10,366 Da
• NCBI Official Full Name: dynein light chain 1, cytoplasmic
• NCBI Official Synonym Full Names: dynein light chain LC8-type 1
• NCBI Official Symbol: Dynll1
• NCBI Official Synonym Symbols: Pin; Dlc8; 8kDLC; Dnclc1
• NCBI Protein Information: dynein light chain 1, cytoplasmic
• UniProt Protein Name: Dynein light chain 1, cytoplasmic
• UniProt Gene Name: Dynll1
• UniProt Synonym Gene Names: Dncl1; Dnclc1; Pin; DLC8; PIN
• UniProt Entry Name: DYL1_RAT

NCBI Description

inhibitor of neuronal nitric oxide synthase, a mediator of apoptosis, synaptogenesis, and neuronal development in the brain [RGD, Feb 2006]

Uniprot Description

LC8: a highly conserved cytoplasmic protein of the dynein light chain family. Cytoplasmic dyneins are large enzyme complexes. They contain two force-producing heads formed primarily from dynein heavy chains, and stalks linking the heads to a basal domain. Interacts with and inhibits the activity of neuronal nitric oxide synthase (nNOS). A highly conserved protein, showing 92% amino acid similarity with the nematode homolog. Binding of this protein destabilizes the nNOS dimer, a conformation necessary for activity, and it may regulate numerous biologic processes through its effects on nitric oxide synthase activity.

Protein type: Motility/polarity/chemotaxis; Microtubule-binding; Motor

Cellular Component: kinetochore; signalosome; microtubule; centrosome; cytoskeleton; mitochondrion; membrane; cytoplasmic dynein complex; cytoplasm; nucleus; cytosol

Molecular Function: protein C-terminus binding; enzyme inhibitor activity; protein domain specific binding; protein binding; protein homodimerization activity; enzyme binding; nitric-oxide synthase regulator activity; motor activity

Biological Process: substantia nigra development; regulation of transcription, DNA-dependent; neurotransmitter metabolic process; transcription, DNA-dependent; regulation of catalytic activity; transport; apoptosis; negative regulation of catalytic activity; negative regulation of phosphorylation; nitric oxide biosynthetic process; microtubule-based process