DAB1 sirna

DAB1 siRNA (Rat)

Cat. Num. AAA8223432

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DAB1; DAB1 siRNA (Rat); Disabled homolog 1; DAB1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: DAB1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 555

• Applicable Applications for DAB1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat DAB1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DAB1 sirna

siRNA to inhibit DAB1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 24025618
• NCBI GeneID: 266729
• NCBI Accession #: NP_705885.1
• NCBI GenBank Nucleotide #: NM_153621.1
• UniProt Accession #: Q8CJH2
• Molecular Weight: 59,900 Da
• NCBI Official Full Name: disabled homolog 1
• NCBI Official Synonym Full Names: Dab, reelin signal transducer, homolog 1 (Drosophila)
• NCBI Official Symbol: Dab1
• NCBI Protein Information: disabled homolog 1
• UniProt Protein Name: Disabled homolog 1
• Protein Family: Disabled
• UniProt Gene Name: Dab1
• UniProt Entry Name: DAB1_RAT

Uniprot Description

DAB1: an adaptor molecule functioning in neural development. The laminar organization of multiple neuronal types in the cerebral cortex is required for normal cognitive function. In mice, the disabled-1 gene plays a central role in brain development, directing the migration of cortical neurons past previously formed neurons to reach their proper layer. Thought to be a signal transducer that interacts with protein kinase pathways to regulate neuronal positioning in the developing brain. Associates with the SH2 domains of Src, Fyn and Abl. Phosphorylated in response to reelin induction in embryonic neurons

Protein type: Adaptor/scaffold

Cellular Component: neuron projection; cell soma; membrane; perinuclear region of cytoplasm; apical part of cell; postsynaptic density; intracellular; cytosol; brush border

Molecular Function: phosphoinositide 3-kinase binding

Biological Process: cell-cell adhesion involved in neuronal-glial interactions involved in cerebral cortex radial glia guided migration; response to drug; cerebellum structural organization; Golgi localization; neuron migration; ventral spinal cord development; negative regulation of cell adhesion; adult walking behavior; cerebral cortex cell migration; positive regulation of protein kinase activity; radial glia guided migration of Purkinje cell; small GTPase mediated signal transduction; negative regulation of astrocyte differentiation; dendrite development; midgut development; negative regulation of axonogenesis; cerebral cortex radially oriented cell migration; brain development; cerebral cortex development; positive regulation of neuron differentiation; negative regulation of JAK-STAT cascade