CYP3A2 sirna

CYP3A2 siRNA (Rat)

Cat. Num. AAA8212479

• Gene Names: Cyp3a2; Cyp3a11
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CYP3A2; CYP3A2 siRNA (Rat); CYP3A-2; CYP3A11; Cytochrome P450 3A2; CYPIIIA2; Cytochrome P450-PCN2; Cytochrome P450/6-beta-A; Testosterone 6-beta-hydroxylase; CYP3A2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: CYP3A2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 504

• Applicable Applications for CYP3A2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CYP3A2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CYP3A2 sirna

siRNA to inhibit CYP3A2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31542329
• NCBI GeneID: 266682
• NCBI Accession #: NP_695224.2
• NCBI GenBank Nucleotide #: NM_153312.2
• UniProt Accession #: P05183; Q5FVU5; Q64629; Q64672
• Molecular Weight: 57,732 Da
• NCBI Official Full Name: cytochrome P450 3A2
• NCBI Official Synonym Full Names: cytochrome P450, family 3, subfamily a, polypeptide 2
• NCBI Official Symbol: Cyp3a2
• NCBI Official Synonym Symbols: Cyp3a11
• NCBI Protein Information: cytochrome P450 3A2
• UniProt Protein Name: Cytochrome P450 3A2
• Protein Family: Cytochrome
• UniProt Gene Name: Cyp3a2
• UniProt Synonym Gene Names: Cyp3a-2; Cyp3a11
• UniProt Entry Name: CP3A2_RAT

NCBI Description

catalyzes the conversion of testosterone to 6-beta-hydroxytestosterone [RGD, Feb 2006]

Uniprot Description

CYP3A4: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4- hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,8-cineole 2- exo-monooxygenase. The enzyme also hydroxylates etoposide. Belongs to the cytochrome P450 family.

Protein type: Cell surface; Endoplasmic reticulum; Oxidoreductase; Membrane protein, peripheral; Demethylase; EC 1.14.14.1; Lipid Metabolism - linoleic acid; Xenobiotic Metabolism - drug metabolism - other enzymes; Cofactor and Vitamin Metabolism - retinol; Membrane protein, integral; Xenobiotic Metabolism - metabolism by cytochrome P450; Xenobiotic Metabolism - drug metabolism - cytochrome P450

Cellular Component: endoplasmic reticulum membrane; cytoplasm

Molecular Function: enzyme binding; iron ion binding; heme binding; steroid hydroxylase activity; oxidoreductase activity; monooxygenase activity; vitamin D3 25-hydroxylase activity; demethylase activity; testosterone 6-beta-hydroxylase activity; steroid binding

Biological Process: steroid metabolic process; drug catabolic process; exogenous drug catabolic process; monoterpenoid metabolic process; alkaloid catabolic process; heterocycle metabolic process; drug metabolic process; steroid catabolic process