CYP1A1 sirna

CYP1A1 siRNA (Rat)

Cat. Num. AAA8202699

• Gene Names: Cyp1a1; AHH; AHRR; CP11; CYP1; Cyp45c; P1-450; P450-C; P450DX; Cypc45c; P-450MC
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CYP1A1; CYP1A1 siRNA (Rat); CYP1A-1; Cytochrome P450 1A1; CYPIA1; Cytochrome P450-C; Cytochrome P450MT2; CYP1A1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: CYP1A1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 524

• Applicable Applications for CYP1A1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CYP1A1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CYP1A1 sirna

siRNA to inhibit CYP1A1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 46048641
• NCBI GeneID: 24296
• NCBI Accession #: NP_036672.2
• NCBI GenBank Nucleotide #: NM_012540.2
• UniProt Accession #: P00185
• Molecular Weight: 59,393 Da
• NCBI Official Full Name: cytochrome P450 1A1
• NCBI Official Synonym Full Names: cytochrome P450, family 1, subfamily a, polypeptide 1
• NCBI Official Symbol: Cyp1a1
• NCBI Official Synonym Symbols: AHH; AHRR; CP11; CYP1; Cyp45c; P1-450; P450-C; P450DX; Cypc45c; P-450MC
• NCBI Protein Information: cytochrome P450 1A1
• UniProt Protein Name: Cytochrome P450 1A1
• Protein Family: Cytochrome
• UniProt Gene Name: Cyp1a1
• UniProt Synonym Gene Names: Cyp1a-1; CYP1A1
• UniProt Entry Name: CP1A1_RAT

NCBI Description

monooxygenase that plays a role in dioxin metabolism and detoxification which is 3-methylcholanthrene-inducible [RGD, Feb 2006]

Uniprot Description

CYP1A1: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics

Protein type: Amino Acid Metabolism - tryptophan; Xenobiotic Metabolism - metabolism by cytochrome P450; Cofactor and Vitamin Metabolism - retinol; EC 1.14.14.1; Oxidoreductase

Cellular Component: endoplasmic reticulum membrane; mitochondrion; intracellular membrane-bound organelle; mitochondrial membrane; cytoplasm

Molecular Function: oxidoreductase activity, acting on diphenols and related substances as donors; flavonoid 3'-monooxygenase activity; protein binding; enzyme binding; iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; steroid hydroxylase activity; oxidoreductase activity; catalytic activity; monooxygenase activity; demethylase activity

Biological Process: coumarin metabolic process; dibenzo-p-dioxin metabolic process; response to nematode; response to arsenic; response to toxin; response to lipopolysaccharide; response to organic cyclic substance; response to vitamin A; response to antibiotic; response to organic substance; embryonic development ending in birth or egg hatching; flavonoid metabolic process; porphyrin metabolic process; response to wounding; heterocycle metabolic process; drug metabolic process; aging; response to drug; response to food; insecticide metabolic process; camera-type eye development; response to iron(III) ion; response to virus; response to herbicide; liver development; dibenzo-p-dioxin catabolic process; cell proliferation; response to hyperoxia; gut development; toxin metabolic process; maternal process involved in parturition; response to hypoxia; 9-cis-retinoic acid biosynthetic process; amine metabolic process; developmental process; hydrogen peroxide biosynthetic process