CRYAB sirna

CRYAB siRNA (Rat)

Cat. Num. AAA8220761

• Gene Names: Cryab; AACRYA
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CRYAB; CRYAB siRNA (Rat); Alpha-crystallin B chain; Alpha(B)-crystallin; CRYAB sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: CRYAB siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 175

• Applicable Applications for CRYAB sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CRYAB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CRYAB sirna

siRNA to inhibit CRYAB expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 16905067
• NCBI GeneID: 25420
• NCBI Accession #: NP_037067.1
• NCBI GenBank Nucleotide #: NM_012935.4
• UniProt Accession #: P23928; Q6LDR2
• Molecular Weight: 20,089 Da
• NCBI Official Full Name: alpha-crystallin B chain
• NCBI Official Synonym Full Names: crystallin, alpha B
• NCBI Official Symbol: Cryab
• NCBI Official Synonym Symbols: AACRYA
• NCBI Protein Information: alpha-crystallin B chain
• UniProt Protein Name: Alpha-crystallin B chain
• Protein Family: Alpha-crystallin
• UniProt Gene Name: Cryab
• UniProt Entry Name: CRYAB_RAT

NCBI Description

This gene encodes subunit b, one of two subunits of alpha-crystallin, which is a high molecular weight, soluble aggregate and is a member of the small heat shock protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. It acts as a molecular chaperone and is the major protein in the eye lens, maintaining the transparency and refractive index of the lens. Alternate promoter usage results in different transcript variants encoding the same protein. [provided by RefSeq, Sep 2014]

Uniprot Description

CRYAB: a major structural protein of the eye lens. A member of the small heat shock protein (sHSP also known as the HSP20) family. Alpha-B is expressed in the lens as well as other tissues. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy.

Protein type: Heat shock protein; Chaperone

Cellular Component: Golgi apparatus; I band; cell surface; mitochondrion; contractile fiber; actin filament bundle; cytosol; Z disc; microtubule cytoskeleton; cytoplasm; plasma membrane; nucleus; myelin sheath

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; metal ion binding; microtubule binding; cytoskeletal protein binding; unfolded protein binding; structural constituent of eye lens

Biological Process: lens development in camera-type eye; muscle development; camera-type eye development; protein folding; stress-activated MAPK cascade; microtubule polymerization or depolymerization; negative regulation of caspase activity; response to estradiol stimulus; tubulin folding; negative regulation of intracellular transport; response to hydrogen peroxide; response to hypoxia; negative regulation of cell growth; protein homooligomerization; negative regulation of apoptosis; aging