CRCP sirna

CRCP siRNA (Rat)

Cat. Num. AAA8205304

• Gene Names: Crcp; Cgrp-rcp
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CRCP; CRCP siRNA (Rat); DNA-directed RNA polymerase III subunit RPC9; RNA polymerase III subunit C9; Calcitonin gene-related peptide-receptor component protein; CGRP-RCP; CGRP-receptor component protein; CGRPRCP; CRCP sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: CRCP siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 148

• Applicable Applications for CRCP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CRCP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CRCP sirna

siRNA to inhibit CRCP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 19526763
• NCBI GeneID: 114205
• NCBI Accession #: NP_446122.1
• NCBI GenBank Nucleotide #: NM_053670.3
• UniProt Accession #: Q8VHM6; Q9Z0W9
• Molecular Weight: 16,627 Da
• NCBI Official Full Name: DNA-directed RNA polymerase III subunit RPC9
• NCBI Official Synonym Full Names: CGRP receptor component
• NCBI Official Symbol: Crcp
• NCBI Official Synonym Symbols: Cgrp-rcp
• NCBI Protein Information: DNA-directed RNA polymerase III subunit RPC9
• UniProt Protein Name: DNA-directed RNA polymerase III subunit RPC9
• UniProt Gene Name: Crcp
• UniProt Synonym Gene Names: RNA polymerase III subunit C9; CGRP-RCP; CGRP-receptor component protein; CGRPRCP
• UniProt Entry Name: RPC9_RAT

NCBI Description

may play a role in a neuroendocrine stress response [RGD, Feb 2006]

Uniprot Description

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts induce type I interferon and NF- Kappa-B through the RIG-I pathway ().