CHM sirna

CHM siRNA (Rat)

Cat. Num. AAA8232517

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CHM; CHM siRNA (Rat); REP1; Rab proteins geranylgeranyltransferase component A 1; Choroideremia protein homolog; Rab escort protein 1; REP-1; CHM sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: CHM siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 650

• Applicable Applications for CHM sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CHM gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CHM sirna

siRNA to inhibit CHM expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 8393110
• NCBI GeneID: 24942
• NCBI Accession #: NP_058763.1
• NCBI GenBank Nucleotide #: NM_017067.1
• UniProt Accession #: P37727
• Molecular Weight: 72,480 Da
• NCBI Official Full Name: rab proteins geranylgeranyltransferase component A 1
• NCBI Official Synonym Full Names: choroideremia (Rab escort protein 1)
• NCBI Official Symbol: Chm
• NCBI Protein Information: rab proteins geranylgeranyltransferase component A 1
• UniProt Protein Name: Rab proteins geranylgeranyltransferase component A 1
• Protein Family: Rab proteins geranylgeranyltransferase component
• UniProt Gene Name: Chm
• UniProt Synonym Gene Names: Rep1; REP-1
• UniProt Entry Name: RAE1_RAT

NCBI Description

may function as Rab escort protein (REP); mutations in human homolog associated with choroideremia, a disease of retinal degeneration [RGD, Feb 2006]

Uniprot Description

CHM: Binds unprenylated Rab proteins, presents it to the catalytic Rab GGTase dimer, and remains bound to it after the geranylgeranyl transfer reaction. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Also a pre-formed complex consisting of CHM and the Rab GGTase dimer (RGGT or component B) can bind to and prenylate Rab proteins; this alternative pathway is proposed to be the predominant pathway for Rab protein geranylgeranylation. Defects in CHM are the cause of choroideremia (CHM). An X-linked recessive disease characterized by a slowly progressive degeneration of the choroid, photoreceptors, and retinal pigment epithelium. Affected males develop night blindness in their teenage years followed by loss of peripheral vision and complete blindness at middle age. Carrier females are generally asymptomatic but funduscopic examination often shows patchy areas of chorioretinal atrophy. Belongs to the Rab GDI family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase

Cellular Component: Rab-protein geranylgeranyltransferase complex; cytosol

Molecular Function: protein binding; GTPase activator activity; Rab GTPase binding; Rab-protein geranylgeranyltransferase activity

Biological Process: protein geranylgeranylation; blood vessel development; protein targeting to membrane; positive regulation of GTPase activity