CDC42 sirna

CDC42 siRNA (Rat)

Cat. Num. AAA8202450

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CDC42; CDC42 siRNA (Rat); Cell division control protein 42 homolog; CDC42 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: CDC42 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 191

• Applicable Applications for CDC42 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CDC42 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CDC42 sirna

siRNA to inhibit CDC42 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 61889112
• NCBI GeneID: 64465
• NCBI Accession #: NP_741991.3
• NCBI GenBank Nucleotide #: NM_171994.4
• UniProt Accession #: Q8CFN2; Q6P9Y3; Q71TW5
• Molecular Weight: 21,283 Da
• NCBI Official Full Name: cell division control protein 42 homolog
• NCBI Official Synonym Full Names: cell division cycle 42
• NCBI Official Symbol: Cdc42
• NCBI Protein Information: cell division control protein 42 homolog
• UniProt Protein Name: Cell division control protein 42 homolog
• Protein Family: Cdc42
• UniProt Gene Name: Cdc42
• UniProt Entry Name: CDC42_RAT

NCBI Description

small GTPase; involved in formation of cellular asymmetry [RGD, Feb 2006]

Uniprot Description

CDC42: a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. Causes the formation of thin, actin-rich surface projections called filopodia. The oncoprotein Dbl specifically catalyzes the dissociation of GDP from this protein. Regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Interacts with DOCK9 which activates it by exchanging GDP for GTP. Interacts with PARD6A, PARD6B and PARD6G in a GTP-dependent manner. Part of a complex with PARD3, PARD6A or PARD6B and PRKCI or PRKCZ. Interacts with CDC42EP4.Alternative splicing of this gene results in at least two transcript variants.

Protein type: G protein; G protein, monomeric, Rho; G protein, monomeric; Motility/polarity/chemotaxis

Cellular Component: neuron projection; focal adhesion; cell; intercellular junction; cytosol; secretory granule; Golgi membrane; cell projection; membrane; cell soma; apical part of cell; cytoplasm; plasma membrane; microtubule organizing center; midbody; spindle midzone; myelin sheath; filopodium

Molecular Function: GTPase activity; identical protein binding; protein binding; GTP binding; GTP-dependent protein binding; thioesterase binding; mitogen-activated protein kinase kinase kinase binding; protein kinase binding; apolipoprotein A-I receptor binding

Biological Process: regulation of protein heterodimerization activity; filopodium formation; metabolic process; regulation of protein metabolic process; positive regulation of JNK cascade; regulation of protein stability; regulation of filopodium formation; Wnt receptor signaling pathway through beta-catenin; cell-cell adhesion; substantia nigra development; positive regulation of MAPKKK cascade; establishment of Golgi localization; small GTPase mediated signal transduction; epidermis morphogenesis; keratinization; positive regulation of neuron apoptosis; response to glucose stimulus; regulation of mitosis; Golgi organization and biogenesis; positive regulation of cytokinesis; neuron fate determination; actin filament bundle formation; regulation of attachment of spindle microtubules to kinetochore; hair follicle morphogenesis; multicellular organism growth; actin filament organization; positive regulation of phosphoinositide 3-kinase activity; endocytosis; establishment and/or maintenance of apical/basal cell polarity; positive regulation of metalloenzyme activity; positive regulation of peptidyl-serine phosphorylation; nucleus localization; positive regulation of synapse structural plasticity; regulation of exocytosis; positive regulation of pseudopodium formation; establishment of cell polarity; heart contraction; regulation of protein catabolic process; sprouting angiogenesis; positive regulation of protein amino acid phosphorylation; actin cytoskeleton organization and biogenesis; regulation of protein kinase activity; negative regulation of protein complex assembly; nuclear migration; positive regulation of DNA replication