CAND1 sirna

CAND1 siRNA (Rat)

Cat. Num. AAA8214432

• Gene Names: Cand1; Tip120; Tip120A
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CAND1; CAND1 siRNA (Rat); TIP120; TIP120A; Cullin-associated NEDD8-dissociated protein 1; Cullin-associated and neddylation-dissociated protein 1; TBP-interacting protein of 120 kDa A; TBP-interacting protein 120A; p120 CAND1; CAND1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: CAND1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1230

• Applicable Applications for CAND1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CAND1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CAND1 sirna

siRNA to inhibit CAND1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 16758920
• NCBI GeneID: 117152
• NCBI Accession #: NP_446456.1
• NCBI GenBank Nucleotide #: NM_054004.2
• UniProt Accession #: P97536
• Molecular Weight: 136,362 Da
• NCBI Official Full Name: cullin-associated NEDD8-dissociated protein 1
• NCBI Official Synonym Full Names: cullin-associated and neddylation-dissociated 1
• NCBI Official Symbol: Cand1
• NCBI Official Synonym Symbols: Tip120; Tip120A
• NCBI Protein Information: cullin-associated NEDD8-dissociated protein 1
• UniProt Protein Name: Cullin-associated NEDD8-dissociated protein 1
• UniProt Gene Name: Cand1
• UniProt Synonym Gene Names: Tip120; Tip120a; TBP-interacting protein 120A
• UniProt Entry Name: CAND1_RAT

NCBI Description

interacts with TATA-binding protein and may be involved in transcription regulation [RGD, Feb 2006]

Uniprot Description

CAND1: Enhances transcription from various types of promoters. Regulatory protein that interferes with the assembly of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex and thereby down-regulates ubiquitination of target proteins. Prevents neddylation of CUL1 by physically blocking access to the neddylation site. Disrupts interactions between CUL1 and SKP1 and between CUL1 and F-box proteins. Binds TBP. Part of a complex that contains CUL1 and RBX1. Binds unneddylated CUL1, but cannot bind CUL1 once it has been neddylated. Binds CUL2, CUL3, CUL4A, CUL4B and CUL5. Belongs to the CAND family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Transcription factor

Cellular Component: nucleoplasm; cullin-RING ubiquitin ligase complex; membrane; cytoplasm; nucleus; ubiquitin ligase complex

Molecular Function: TATA-binding protein binding

Biological Process: transcription, DNA-dependent; SCF complex assembly; positive regulation of transcription, DNA-dependent; negative regulation of catalytic activity; protein ubiquitination; positive regulation of transcriptional preinitiation complex assembly; cell differentiation