BATF sirna

BATF siRNA (Rat)

Cat. Num. AAA8206761

• Gene Names: Batf; B-ATF
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BATF; BATF siRNA (Rat); Basic leucine zipper transcriptional factor ATF-like; B-cell-activating transcription factor; B-ATF; BATF sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: BATF siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 125

• Applicable Applications for BATF sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat BATF gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BATF sirna

siRNA to inhibit BATF expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 157822617
• NCBI GeneID: 299206
• NCBI Accession #: NP_001100218.1
• NCBI GenBank Nucleotide #: NM_001106748.1
• UniProt Accession #: D4A7E1
• Molecular Weight: 14,065 Da
• NCBI Official Full Name: basic leucine zipper transcriptional factor ATF-like
• NCBI Official Synonym Full Names: basic leucine zipper transcription factor, ATF-like
• NCBI Official Symbol: Batf
• NCBI Official Synonym Symbols: B-ATF
• NCBI Protein Information: basic leucine zipper transcriptional factor ATF-like
• UniProt Protein Name: Basic leucine zipper transcriptional factor ATF-like
• Protein Family: Basic leucine zipper transcriptional factor
• UniProt Gene Name: Batf
• UniProt Synonym Gene Names: B-ATF
• UniProt Entry Name: BATF_RAT

Uniprot Description

AP-1 family transcription factor that controls the differentiation of lineage-specific cells in the immune system: specifically mediates the differentiation of T-helper 17 cells (Th17), follicular T-helper cells (TfH), CD8+ dendritic cells and class-switch recombination (CSR) in B-cells. Acts via the formation of a heterodimer with JUNB that recognizes and binds DNA sequence 5'-TGA[CG]TCA-3'. The BATF-JUNB heterodimer also forms a complex with IRF4 (or IRF8) in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 (or IRF8) and activation of genes. Controls differentiation of T-helper cells producing interleukin-17 (Th17 cells) by binding to Th17-associated gene promoters: regulates expression of the transcription factor RORC itself and RORC target genes such as IL17 (IL17A or IL17B). Also involved in differentiation of follicular T-helper cells (TfH) by directing expression of BCL6 and MAF. In B-cells, involved in class-switch recombination (CSR) by controlling the expression of both AICDA and of germline transcripts of the intervening heavy-chain region and constant heavy-chain region (I(H)-C(H)). Following infection, can participate in CD8+ dendritic cell differentiation via interaction with IRF4 and IRF8 to mediate cooperative gene activation. Regulates effector CD8+ T-cell differentiation by regulating expression of SIRT1. Following DNA damage, part of a differentiation checkpoint that limits self-renewal of hematopoietic stem cells (HSCs): up-regulated by STAT3, leading to differentiation of HSCs, thereby restricting self-renewal of HSCs ().