ARRB1 sirna

ARRB1 siRNA (Rat)

Cat. Num. AAA8240359

• Gene Names: Arrb1; BARRES
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ARRB1; ARRB1 siRNA (Rat); Beta-arrestin-1; Arrestin beta-1; ARRB1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: ARRB1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 418

• Applicable Applications for ARRB1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ARRB1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ARRB1 sirna

siRNA to inhibit ARRB1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6978537
• NCBI GeneID: 25387
• NCBI Accession #: NP_037042.1
• NCBI GenBank Nucleotide #: NM_012910.2
• UniProt Accession #: P29066
• Molecular Weight: 47,020 Da
• NCBI Official Full Name: beta-arrestin-1
• NCBI Official Synonym Full Names: arrestin, beta 1
• NCBI Official Symbol: Arrb1
• NCBI Official Synonym Symbols: BARRES
• NCBI Protein Information: beta-arrestin-1
• UniProt Protein Name: Beta-arrestin-1
• Protein Family: Beta-arrestin
• UniProt Gene Name: Arrb1
• UniProt Entry Name: ARRB1_RAT

Uniprot Description

ARRB1: regulates G-protein coupled receptors (GPCR) signaling by mediating both receptor desensitization and resensitization processes. Binds to GRK-phosphorylated receptor and sterically preclude its coupling to the cognate G- protein; the binding appears to require receptor determinants exposed only in the active receptor conformation. Targets many receptors for internalization by acting as endocytic adapters (CLASPs, clathrin-associated sorting proteins). Internalized arrestin-receptor complexes traffic to intracellular endosomes, where they remain uncoupled from G-proteins. Two different modes of arrestin-mediated internalization occur. Beta-arrestins function as multivalent adapter proteins that can switch the GPCR from a G-protein signaling mode that transmits short-lived signals from the plasma membrane via small molecule second messengers and ion channels to a beta-arrestin signaling mode that transmits a distinct set of signals that are initiated as the receptor internalizes and transits the intracellular compartment. Also involved in regulation of receptors other than GPCRs. Involved in Toll-like receptor and IL-1 receptor signaling through the interaction with TRAF6 which prevents TRAF6 autoubiquitination and oligomerization required for activation of NF-kappa-B and JUN. Binds phosphoinositides. Binds inositolhexakisphosphate (InsP6). Involved in IL8-mediated granule release in neutrophils. Interacts with phosphorylated ADRB2 and CHRM2. Interacts with SRC (via the SH3 domain and the protein kinase domain); the interaction is independent of the phosphorylation state of SRC C-terminus. Interacts with RAF1, CHUK, IKBKB and Nik. Interacts with DVL1 and DVL2; the interaction is enhanced by DVL phosphorylation. Interacts with IGF1R. Belongs to the arrestin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Cellular Component: basolateral plasma membrane; postsynaptic density; cytoplasmic membrane-bound vesicle; dendritic spine; coated pit; pseudopodium; cytosol; nucleoplasm; postsynaptic membrane; cytoplasm; plasma membrane; cytoplasmic vesicle; intracellular; chromatin; nucleus

Molecular Function: angiotensin receptor binding; protein phosphorylated amino acid binding; clathrin binding; mitogen-activated protein kinase kinase binding; follicle stimulating hormone receptor binding; caspase inhibitor activity; phosphoprotein binding; alpha-1B adrenergic receptor binding; transcription factor binding; GTPase activator activity; alpha-1A adrenergic receptor binding; V2 vasopressin receptor binding; insulin-like growth factor receptor binding; protein binding; histone acetyltransferase activity; G-protein-coupled receptor binding; ubiquitin protein ligase binding; estrogen receptor binding

Biological Process: transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; follicle-stimulating hormone signaling pathway; positive regulation of protein binding; activation of MAPK activity; positive regulation of receptor internalization; protein ubiquitination; positive regulation of caspase activity; negative regulation of caspase activity; sensory perception of pain; protein transport; negative regulation of protein amino acid phosphorylation; sensory perception of touch; phototransduction; negative regulation of interleukin-8 production; negative regulation of interleukin-6 production; regulation of cAMP biosynthetic process; arrestin mediated desensitization of G-protein coupled receptor protein signaling pathway; receptor-mediated endocytosis; positive regulation of Rho protein signal transduction; endocytosis; regulation of cAMP catabolic process; positive regulation of peptidyl-serine phosphorylation; G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein coupled receptor protein signaling pathway; G-protein coupled receptor internalization; positive regulation of protein ubiquitination; inhibition of NF-kappaB transcription factor; stress fiber formation; positive regulation of histone acetylation; negative regulation of protein ubiquitination; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; regulation of G-protein coupled receptor protein signaling pathway; positive regulation of GTPase activity