APC sirna

APC siRNA (Rat)

Cat. Num. AAA8218625

• Gene Names: Apc; RATAPC
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: APC; APC siRNA (Rat); Adenomatous polyposis coli protein; Protein APC; APC sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: APC siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2842

• Applicable Applications for APC sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat APC gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for APC sirna

siRNA to inhibit APC expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6978509
• NCBI GeneID: 24205
• NCBI Accession #: NP_036631.1
• NCBI GenBank Nucleotide #: NM_012499.1
• UniProt Accession #: P70478
• Molecular Weight: 310,533 Da
• NCBI Official Full Name: adenomatous polyposis coli protein
• NCBI Official Synonym Full Names: adenomatous polyposis coli
• NCBI Official Symbol: Apc
• NCBI Official Synonym Symbols: RATAPC
• NCBI Protein Information: adenomatous polyposis coli protein
• UniProt Protein Name: Adenomatous polyposis coli protein
• Protein Family: APC-related protein
• UniProt Gene Name: Apc
• UniProt Synonym Gene Names: Protein APC
• UniProt Entry Name: APC_RAT

NCBI Description

binds microtubules; may play a role in the regulation of cell polarity [RGD, Feb 2006]

Uniprot Description

APC: a tumor suppressor. Defects in APC are a cause of familial adenomatous polyposis (FAP). Promotes rapid degradation of CTNNB1 and participates in Wnt signaling. Activity is correlated with its phosphorylation state. Two splice-variant isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Tumor suppressor

Cellular Component: microtubule; centrosome; neuron projection; nuclear membrane; protein complex; tight junction; cell; dendrite; cell cortex; beta-catenin destruction complex; kinetochore; cell projection; growth cone; cell-cell adherens junction; cell soma; cytoplasmic microtubule; axon; cell projection membrane; lamellipodium; cytoplasm; plasma membrane; synapse; nucleus; lateral plasma membrane

Molecular Function: microtubule plus-end binding; protein binding; protease binding; cadherin binding; gamma-catenin binding; microtubule binding; protein complex binding; beta-catenin binding; protein kinase regulator activity; protein kinase binding

Biological Process: skin development; positive regulation of cell adhesion; negative regulation of Wnt receptor signaling pathway; positive regulation of apoptosis; somatic stem cell maintenance; regulation of cell cycle; T cell differentiation in the thymus; Wnt receptor signaling pathway through beta-catenin; regulation of cell migration; cytoplasmic microtubule organization and biogenesis; anterior/posterior pattern formation; muscle maintenance; cytokinesis after mitosis; hair follicle development; positive regulation of epithelial cell differentiation; protein complex assembly; kidney development; cell cycle arrest; neurite development; response to drug; regulation of microtubule-based process; negative regulation of odontogenesis; regulation of osteoblast differentiation; negative regulation of cyclin-dependent protein kinase activity; retina development in camera-type eye; positive regulation of cell division; regulation of epithelial cell differentiation; positive regulation of protein catabolic process; regulation of nitrogen compound metabolic process; positive regulation of cell differentiation; mitotic metaphase/anaphase transition; negative regulation of apoptosis; positive regulation of microtubule polymerization; establishment and/or maintenance of cell polarity; chromosome organization and biogenesis; negative regulation of cell proliferation; regulation of osteoclast differentiation; regulation of cell differentiation; pancreas development; mitotic cell cycle spindle assembly checkpoint; proximal/distal pattern formation; negative regulation of epithelial cell proliferation; cell migration; regulation of attachment of spindle microtubules to kinetochore; Wnt receptor signaling pathway; thymus development; negative regulation of microtubule depolymerization; negative regulation of MAPKKK cascade; axis specification; stem cell maintenance; pattern specification process; positive regulation of pseudopodium formation; dorsal/ventral pattern formation; axonogenesis; response to DNA damage stimulus; positive regulation of cell migration