ALDOC sirna

ALDOC siRNA (Rat)

Cat. Num. AAA8217719

• Gene Names: Aldoc; ALDCAA; F16dip7; RATALDCAA
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ALDOC; ALDOC siRNA (Rat); Fructose-bisphosphate aldolase C; Brain-type aldolase; ALDOC sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: ALDOC siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 363

• Applicable Applications for ALDOC sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ALDOC gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ALDOC sirna

siRNA to inhibit ALDOC expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6978489
• NCBI GeneID: 24191
• NCBI Accession #: NP_036629.1
• NCBI GenBank Nucleotide #: NM_012497.1
• UniProt Accession #: P09117; Q54AI4; Q63037
• Molecular Weight: 39,284 Da
• NCBI Official Full Name: fructose-bisphosphate aldolase C
• NCBI Official Synonym Full Names: aldolase C, fructose-bisphosphate
• NCBI Official Symbol: Aldoc
• NCBI Official Synonym Symbols: ALDCAA; F16dip7; RATALDCAA
• NCBI Protein Information: fructose-bisphosphate aldolase C
• UniProt Protein Name: Fructose-bisphosphate aldolase C
• Protein Family: Fructose-bisphosphate aldolase
• UniProt Gene Name: Aldoc
• UniProt Entry Name: ALDOC_RAT

NCBI Description

brain-specific member of the aldolase enzyme family; may play a role in neurogenesis [RGD, Feb 2006]

Uniprot Description

ALDOC: a member of the class I fructose-biphosphate aldolase gene family. Expressed specifically in the hippocampus and Purkinje cells of the brain, the encoded protein is a glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose-1,6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehyde, respectively. [provided by RefSeq, Jul 2008]

Protein type: Carbohydrate Metabolism - pentose phosphate pathway; Lyase; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.1.2.13; Carbohydrate Metabolism - fructose and mannose

Cellular Component: mitochondrion; axon; cytoplasm

Molecular Function: protein binding; cytoskeletal protein binding; fructose-bisphosphate aldolase activity

Biological Process: fructose 1,6-bisphosphate metabolic process; organ regeneration; glycolysis; epithelial cell differentiation; apoptosis; response to hypoxia; protein heterotetramerization; response to organic nitrogen; response to organic cyclic substance; protein homotetramerization; aging