AK2 sirna

AK2 siRNA (Rat)

Cat. Num. AAA8219087

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AK2; AK2 siRNA (Rat); Adenylate kinase 2 mitochondrial; AK 2; ATP-AMP transphosphorylase 2; ATP:AMP phosphotransferase; Adenylate monophosphate kinase; AK2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: AK2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 232

• Applicable Applications for AK2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat AK2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AK2 sirna

siRNA to inhibit AK2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 77020256
• NCBI GeneID: 24184
• NCBI Accession #: NP_001029139.1
• NCBI GenBank Nucleotide #: NM_001033967.2
• UniProt Accession #: P29410; Q6P7C6
• Molecular Weight: 25,530 Da
• NCBI Official Full Name: adenylate kinase 2, mitochondrial isoform b
• NCBI Official Synonym Full Names: adenylate kinase 2
• NCBI Official Symbol: Ak2
• NCBI Protein Information: adenylate kinase 2, mitochondrial
• UniProt Protein Name: Adenylate kinase 2, mitochondrial
• UniProt Gene Name: Ak2
• UniProt Synonym Gene Names: AK 2
• UniProt Entry Name: KAD2_RAT

Uniprot Description

AK2: Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. This small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth. Plays a key role in hematopoiesis. Defects in AK2 are the cause of reticular dysgenesis (RDYS); also known as aleukocytosis. RDYS is the most severe form of inborn severe combined immunodeficiencies (SCID) and is characterized by absence of granulocytes and almost complete deficiency of lymphocytes in peripheral blood, hypoplasia of the thymus and secondary lymphoid organs, and lack of innate and adaptive humoral and cellular immune functions, leading to fatal septicemia within days after birth. In bone marrow of individuals with reticular dysgenesis, myeloid differentiation is blocked at the promyelocytic stage, whereas erythro- and megakaryocytic maturation is generally normal.In addition, affected newborns have bilateral sensorineural deafness. Defects may be due to its absence in leukocytes and inner ear, in which its absence can not be compensated by AK1. Belongs to the adenylate kinase family. AK2 subfamily. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleotide Metabolism - purine; Kinase, other; Mitochondrial; EC 2.7.4.3

Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial intermembrane space; cytosol

Molecular Function: nucleobase, nucleoside, nucleotide kinase activity; adenylate kinase activity; ATP binding

Biological Process: ATP metabolic process; dATP metabolic process; ADP biosynthetic process; brain development; liver development; AMP metabolic process; adenine metabolic process; oxidative phosphorylation; nucleotide phosphorylation