AHI1 sirna

AHI1 siRNA (Rat)

Cat. Num. AAA828145

• Gene Names: Ahi1; Ahi-1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AHI1; AHI1 siRNA (Rat); Jouberin; Abelson helper integration site 1 protein homolog; AHI-1; AHI1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: AHI1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,047

• Applicable Applications for AHI1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat AHI1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AHI1 sirna

siRNA to inhibit AHI1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 50511316
• NCBI GeneID: 308923
• NCBI Accession #: NP_001002277.1
• NCBI GenBank Nucleotide #: NM_001002277.1
• UniProt Accession #: Q6DTM3
• Molecular Weight: 119,687 Da
• NCBI Official Full Name: jouberin
• NCBI Official Synonym Full Names: Abelson helper integration site 1
• NCBI Official Symbol: Ahi1
• NCBI Official Synonym Symbols: Ahi-1
• NCBI Protein Information: jouberin
• UniProt Protein Name: Jouberin
• Protein Family: Jouberin
• UniProt Gene Name: Ahi1
• UniProt Synonym Gene Names: AHI-1
• UniProt Entry Name: AHI1_RAT

NCBI Description

mutations in the human homolog are associated with Joubert syndrome, an autosomal recessive disorder resulting in severe mental retardation [RGD, Feb 2006]

Uniprot Description

AHI1: Component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes (By similarity)

Protein type: Adaptor/scaffold

Cellular Component: centriole; centrosome; photoreceptor outer segment; adherens junction; nonmotile primary cilium; intercellular junction; cilium

Molecular Function: identical protein binding

Biological Process: positive regulation of polarized epithelial cell differentiation; central nervous system development; vesicle targeting; positive regulation of receptor internalization; specification of axis polarity; vesicle-mediated transport; retina development in camera-type eye; protein localization in organelle; cilium biogenesis; positive regulation of transcription from RNA polymerase II promoter; heart looping; transmembrane receptor protein tyrosine kinase signaling pathway; morphogenesis of a polarized epithelium; regulation of behavior; hindbrain development; negative regulation of apoptosis