ADARB1 sirna

ADARB1 siRNA (Rat)

Cat. Num. AAA8227635

• Gene Names: Adarb1; Red1; Adar2
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ADARB1; ADARB1 siRNA (Rat); RED1; Double-stranded RNA-specific editase 1; RNA-editing deaminase 1; RNA-editing enzyme 1; dsRNA adenosine deaminase; ADARB1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: ADARB1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 711

• Applicable Applications for ADARB1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ADARB1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ADARB1 sirna

siRNA to inhibit ADARB1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 161377409
• NCBI GeneID: 25367
• NCBI Accession #: NP_001104525.1
• NCBI GenBank Nucleotide #: NM_001111055.1
• UniProt Accession #: P51400
• Molecular Weight: 77,925 Da
• NCBI Official Full Name: double-stranded RNA-specific editase 1 isoform 2
• NCBI Official Synonym Full Names: adenosine deaminase, RNA-specific, B1
• NCBI Official Symbol: Adarb1
• NCBI Official Synonym Symbols: Red1; Adar2
• NCBI Protein Information: double-stranded RNA-specific editase 1
• UniProt Protein Name: Double-stranded RNA-specific editase 1
• Protein Family: Double-stranded RNA-specific editase
• UniProt Gene Name: Adarb1
• UniProt Synonym Gene Names: Red1
• UniProt Entry Name: RED1_RAT

NCBI Description

This gene encodes a double-stranded-RNA-specific adenosine deaminase that is involved in editing pre-mRNAs by site-specific conversion of adenosine (A) to inosine (I). Substrates for this enzyme include ionotropic glutamate receptors (GluR2-6) and serotonin receptor (5HT2C). Studies in rodents have shown that this protein can modify its own pre-mRNA by A->I editing to create a novel acceptor splice site, alternative splicing to which results in down regulation of its protein expression. Additional splicing events result in transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2 and GRIK2) and serotonin (HTR2C), GABA receptor (GABRA3) and potassium voltage-gated channel (KCNA1). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alter their functional activities. Edits GRIA2 at both the Q/R and R/G sites efficiently but converts the adenosine in hotspot1 much less efficiently (). Can inhibit cell proliferation and migration and can stimulate exocytosis.