ACVR2B sirna

ACVR2B siRNA (Rat)

Cat. Num. AAA8240106

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ACVR2B; ACVR2B siRNA (Rat); ACTRIIB; Activin receptor type-2B; Activin receptor type IIB; ACTR-IIB; ACVR2B sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: ACVR2B siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 512

• Applicable Applications for ACVR2B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ACVR2B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ACVR2B sirna

siRNA to inhibit ACVR2B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 67514518
• NCBI GeneID: 25366
• NCBI Accession #: NP_113742.1
• NCBI GenBank Nucleotide #: NM_031554.1
• UniProt Accession #: P38445; Q4V8J8
• Molecular Weight: 57,857 Da
• NCBI Official Full Name: activin receptor type-2B
• NCBI Official Synonym Full Names: activin A receptor, type IIB
• NCBI Official Symbol: Acvr2b
• NCBI Protein Information: activin receptor type-2B
• UniProt Protein Name: Activin receptor type-2B
• Protein Family: Activin receptor
• UniProt Gene Name: Acvr2b
• UniProt Synonym Gene Names: Actriib; ACTR-IIB
• UniProt Entry Name: AVR2B_RAT

NCBI Description

plays a role in reproductive physiology and endocrinology [RGD, Feb 2006]

Uniprot Description

ACVR2B: a tyrosine-kinase like receptor kinase of the STKR family. The holoreceptor receptor is a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. Each is composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic kinase domain. Type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptor kinases are apparently constitutively active. The IIB receptor displays a 3- to 4-fold higher affinity for activin A than the II receptor.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (receptor); Membrane protein, integral; EC 2.7.11.30; Protein kinase, TKL; TKL group; STKR family; Type2 subfamily

Cellular Component: protein complex; cell; cytoplasm; integral to membrane; plasma membrane; receptor complex

Molecular Function: protein serine/threonine kinase activity; transforming growth factor beta binding; growth factor binding; metal ion binding; protein serine/threonine/tyrosine kinase activity; activin binding; ATP binding; transmembrane receptor protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: heart development; regulation of signal transduction; palate development; signal transduction; protein amino acid phosphorylation; post-embryonic development; anterior/posterior pattern formation; BMP signaling pathway; positive regulation of activin receptor signaling pathway; regulation of transcription, DNA-dependent; lymphangiogenesis; pancreas development; embryonic foregut morphogenesis; response to glucose stimulus; mesoderm development; kidney development; skeletal development; gastrulation with mouth forming second; positive regulation of bone mineralization; pattern specification process; odontogenesis of dentine-containing teeth; organ growth; positive regulation of osteoblast differentiation; skeletal morphogenesis; insulin secretion; sexual reproduction; activation of protein kinase activity; blood vessel remodeling; determination of left/right symmetry; lung development