ACTN4 sirna

ACTN4 siRNA (Rat)

Cat. Num. AAA8207129

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ACTN4; ACTN4 siRNA (Rat); Alpha-actinin-4; F-actin cross-linking protein; Non-muscle alpha-actinin 4; ACTN4 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: ACTN4 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 911

• Applicable Applications for ACTN4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ACTN4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ACTN4 sirna

siRNA to inhibit ACTN4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 77539778
• NCBI GeneID: 63836
• NCBI Accession #: NP_113863.2
• NCBI GenBank Nucleotide #: NM_031675.2
• UniProt Accession #: Q9QXQ0; Q6P786
• Molecular Weight: 104,915 Da
• NCBI Official Full Name: alpha-actinin-4
• NCBI Official Synonym Full Names: actinin alpha 4
• NCBI Official Symbol: Actn4
• NCBI Protein Information: alpha-actinin-4
• UniProt Protein Name: Alpha-actinin-4
• Protein Family: Alpha-actinin
• UniProt Gene Name: Actn4
• UniProt Entry Name: ACTN4_RAT

NCBI Description

alpha-actinin cytoskeletal protein that anchors various actin-containing microfilaments to cellular structures; anchors V myosins to cellular structures via the RING finger-containing protein BERP [RGD, Feb 2006]

Uniprot Description

ACTN4: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Defects in ACTN4 are the cause of focal segmental glomerulosclerosis type 1 (FSGS1). A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. Belongs to the alpha-actinin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Cellular Component: cortical cytoskeleton; extracellular space; neuron projection; focal adhesion; protein complex; pseudopodium; intercellular junction; Z disc; cytosol; ribonucleoprotein complex; perinuclear region of cytoplasm; cytoplasm; stress fiber; nucleus

Molecular Function: actin filament binding; protein binding; protein homodimerization activity; ligand-dependent nuclear receptor transcription coactivator activity; retinoic acid receptor binding; nucleoside binding; protein complex binding; protein N-terminus binding; calcium ion binding; nuclear hormone receptor binding

Biological Process: retinoic acid receptor signaling pathway; regulation of apoptosis; actin filament bundle formation; protein transport; response to hypoxia; negative regulation of cell motility; positive regulation of cell motility; positive regulation of pinocytosis