ZFPM2 sirna

ZFPM2 siRNA (Mouse)

Cat. Num. AAA8203182

• Gene Names: Zfpm2; FOG2; FOG-2; B330005D23Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ZFPM2; ZFPM2 siRNA (Mouse); FOG2; Zinc finger protein ZFPM2; Friend of GATA protein 2; FOG-2; Friend of GATA 2; mFOG-2; Zinc finger protein multitype 2; ZFPM2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ZFPM2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1151

• Applicable Applications for ZFPM2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ZFPM2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ZFPM2 sirna

siRNA to inhibit ZFPM2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6756071
• NCBI GeneID: 22762
• NCBI Accession #: NP_035896.1
• NCBI GenBank Nucleotide #: NM_011766.5
• UniProt Accession #: Q8CCH7; Q9Z0F2
• Molecular Weight: 125,621 Da
• NCBI Official Full Name: zinc finger protein ZFPM2
• NCBI Official Synonym Full Names: zinc finger protein, multitype 2
• NCBI Official Symbol: Zfpm2
• NCBI Official Synonym Symbols: FOG2; FOG-2; B330005D23Rik
• NCBI Protein Information: zinc finger protein ZFPM2
• UniProt Protein Name: Zinc finger protein ZFPM2
• Protein Family: Zinc finger protein
• UniProt Gene Name: Zfpm2
• UniProt Synonym Gene Names: Fog2; FOG-2; Friend of GATA 2; mFOG-2
• UniProt Entry Name: FOG2_MOUSE

Uniprot Description

FOG2: Transcription regulator that plays a central role in heart morphogenesis and development of coronary vessels from epicardium, by regulating genes that are essential during cardiogenesis. Essential cofactor that acts via the formation of a heterodimer with transcription factors of the GATA family GATA4, GATA5 and GATA6. Such heterodimer can both activate or repress transcriptional activity, depending on the cell and promoter context. Also required in gonadal differentiation, possibly be regulating expression of SRY. Probably acts a corepressor of NR2F2. Defects in ZFPM2 may be a cause of tetralogy of Fallot (TOF). TOF is a congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation. Surgical correction is emergent. Defects in ZFPM2 are the cause of diaphragmatic hernia 3 (DIH3); a form of congenital diaphragmatic hernia (CDH). CDH refers to a group of congenital defects in the structural integrity of the diaphragm associated with often lethal pulmonary hypoplasia and pulmonary hypertension. Belongs to the FOG (Friend of GATA) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; C2H2-type zinc finger protein

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; nucleic acid binding; DNA binding; metal ion binding; transcription factor binding; transcription corepressor activity

Biological Process: cardiac muscle development; transcription from RNA polymerase II promoter; transcription, DNA-dependent; in utero embryonic development; heart development; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; negative regulation of fat cell differentiation; regulation of transcription, DNA-dependent; cardiac muscle morphogensis; embryonic organ development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; vasculogenesis; lung development