ZFP36 sirna

ZFP36 siRNA (Mouse)

Cat. Num. AAA8216245

• Gene Names: Zfp36; Ttp; Gos24; TISII; Tis11; Nup475; TIS11D; Zfp-36
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ZFP36; ZFP36 siRNA (Mouse); TIS11; TIS11A; Tristetraprolin; TTP; Growth factor-inducible nuclear protein NUP475; Protein TIS11A; TPA-induced sequence 11; Zinc finger protein 36; Zfp-36; ZFP36 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ZFP36 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 319

• Applicable Applications for ZFP36 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommend transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ZFP36 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4°C. Store at -20°C for one year.

Related Product Information for ZFP36 sirna

siRNA to inhibit ZFP36 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6756059
• NCBI GeneID: 22695
• NCBI Accession #: NP_035886.1
• NCBI GenBank Nucleotide #: NM_011756.4
• UniProt Accession #: P22893; P11520
• NCBI Official Full Name: tristetraprolin
• NCBI Official Synonym Full Names: zinc finger protein 36
• NCBI Official Symbol: Zfp36
• NCBI Official Synonym Symbols: Ttp; Gos24; TISII; Tis11; Nup475; TIS11D; Zfp-36
• NCBI Protein Information: tristetraprolin
• UniProt Protein Name: Tristetraprolin
• Protein Family: mRNA decay activator protein
• UniProt Gene Name: Zfp36
• UniProt Synonym Gene Names: Tis11; Tis11a; TTP; TIS11; Zfp-36
• UniProt Entry Name: TTP_MOUSE

Uniprot Description

TTP: a CCCH zinc finger protein that has anti-inflammatory and arthritis suppressor activity. Regulates inflammatory responses at the post-transcriptional level. TTP deficiency in mice causes a severe inflammatory syndrome that includes autoimmunity, myeloid hyperplasia, and erosive arthritis. Binds to and destabilizes mRNA molecules with AU-rich elements (AREs). Has a high affinity for UUAUUUAUU mRNA sequences. Destabilizes tumor necrosis factor-alpha (TNF??), interleukin 2 (IL2), granulocyte-macrophage colony-stimulating factor (GM-CSF), transcription factor E47, and cyclooxygenase 2 (COX2) mRNAs. TNF?? and GM-CSF mRNAs are stabilized in TTP-deficient mice, causing a severe inflammatory syndrome. Conversely, high levels of TTP reduce inflammatory responses. TTP is a very low abundance protein that is rapidly induced by insulin, glucocorticoids, lipopolysaccharide (LPS), fetal calf serum, zinc, herpes simplex virus 1, green tea, and cinnamon. Once induced, the TTP protein is relatively stable. Has been experimentaly shown to be able to bind zinc. TTP is extensively phosphorylated.

Protein type: RNA-binding; DNA-binding

Cellular Component: stress granule; cytoplasm; nucleus; cytosol

Molecular Function: mRNA binding; protein binding; enzyme binding; DNA binding; metal ion binding; C-C chemokine binding; AU-rich element binding; protein kinase binding

Biological Process: regulation of transcription from RNA polymerase II promoter; response to starvation; poly(A) tail shortening; negative regulation of myeloid cell differentiation; negative regulation of inflammatory response; mRNA catabolic process; miRNA-mediated gene silencing, negative regulation of translation; regulation of tumor necrosis factor production; negative regulation of transcription from RNA polymerase II promoter; RNA destabilization; regulation of mRNA stability; mRNA catabolic process, deadenylation-dependent decay