WRN sirna

WRN siRNA (Mouse)

Cat. Num. AAA8204092

• Gene Names: Wrn; AI846146
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: WRN; WRN siRNA (Mouse); Werner syndrome ATP-dependent helicase homolog; Exonuclease WRN; WRN sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: WRN siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1401

• Applicable Applications for WRN sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse WRN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for WRN sirna

siRNA to inhibit WRN expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 170763504
• NCBI GeneID: 22427
• NCBI Accession #: NP_001116294.1
• NCBI GenBank Nucleotide #: NM_001122822.1
• UniProt Accession #: O09053; O09050; Q80YP9; Q9JKD4; Q9Z241; Q9Z242
• Molecular Weight: 157,204 Da
• NCBI Official Full Name: Werner syndrome ATP-dependent helicase homolog
• NCBI Official Synonym Full Names: Werner syndrome homolog (human)
• NCBI Official Symbol: Wrn
• NCBI Official Synonym Symbols: AI846146
• NCBI Protein Information: Werner syndrome ATP-dependent helicase homolog
• UniProt Protein Name: Werner syndrome ATP-dependent helicase homolog
• Protein Family: Werner syndrome ATP-dependent helicase
• UniProt Gene Name: Wrn
• UniProt Entry Name: WRN_MOUSE

Uniprot Description

WRN: Multifunctional enzyme that has both magnesium and ATP- dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double- stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A. Monomer, and homooligomer. May exist as homodimer, homotrimer, homotetramer and/or homohexamer. Homotetramer, or homohexamer, when bound to DNA. Interacts via its N-terminal domain with WRNIP1. Interacts with EXO1, PCNA and SUPV3L1. Belongs to the helicase family. RecQ subfamily.

Protein type: Helicase; DNA repair, damage; DNA-binding; EC 3.6.4.12; Nucleolus; Cell cycle regulation

Cellular Component: MutLalpha complex; nucleoplasm; centrosome; nucleolus; intracellular; nucleus

Molecular Function: G-quadruplex DNA binding; protein homodimerization activity; metal ion binding; ATPase activity; magnesium ion binding; 3'-5' DNA helicase activity; bubble DNA binding; Y-form DNA binding; four-way junction helicase activity; nucleic acid binding; ATP-dependent 3'-5' DNA helicase activity; exonuclease activity; protein complex binding; 3'-5' exonuclease activity; catalytic activity; ATP binding; nuclease activity; hydrolase activity; nucleotide binding; ATP-dependent helicase activity; helicase activity; T/G mismatch-specific endonuclease activity; phosphoric ester hydrolase activity; ATP-dependent DNA helicase activity; protein binding; DNA helicase activity; DNA binding; manganese ion binding

Biological Process: nucleolus to nucleoplasm transport; metabolic process; positive regulation of hydrolase activity; cell aging; multicellular organismal aging; cellular response to starvation; replicative cell aging; DNA repair; response to UV-C; DNA duplex unwinding; DNA recombination; replication fork processing; regulation of apoptosis; cellular metabolic process; DNA synthesis during DNA repair; base-excision repair; double-strand break repair; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; regulation of growth rate; response to oxidative stress; DNA replication; telomere maintenance; response to DNA damage stimulus; DNA metabolic process