WNT3A sirna

WNT3A siRNA (Mouse)

Cat. Num. AAA8221686

• Gene Names: Wnt3a; vt; Wnt-3a
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: WNT3A; WNT3A siRNA (Mouse); WNT-3A; Protein Wnt-3a; WNT3A sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: WNT3A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 352

• Applicable Applications for WNT3A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse WNT3A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for WNT3A sirna

siRNA to inhibit WNT3A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7106447
• NCBI GeneID: 22416
• NCBI Accession #: NP_033548.1
• NCBI GenBank Nucleotide #: NM_009522.2
• UniProt Accession #: P27467
• Molecular Weight: 39,258 Da
• NCBI Official Full Name: protein Wnt-3a
• NCBI Official Synonym Full Names: wingless-type MMTV integration site family, member 3A
• NCBI Official Symbol: Wnt3a
• NCBI Official Synonym Symbols: vt; Wnt-3a
• NCBI Protein Information: protein Wnt-3a
• UniProt Protein Name: Protein Wnt-3a
• UniProt Gene Name: Wnt3a
• UniProt Synonym Gene Names: Wnt-3a
• UniProt Entry Name: WNT3A_MOUSE

Uniprot Description

WNT3A: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Interacts with PORCN. Interacts with APCDD1 and WLS. Component of the Wnt-Fzd-LRP5-LRP6 signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts directly in the complex with LRP6. Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. Belongs to the Wnt family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; proteinaceous extracellular matrix; cell surface; extracellular region

Molecular Function: protein domain specific binding; protein binding; frizzled binding; transcription coactivator activity; receptor agonist activity; receptor binding; frizzled-2 binding

Biological Process: positive regulation of mesodermal cell fate specification; axon guidance; extracellular matrix organization and biogenesis; positive regulation of protein binding; cell proliferation in forebrain; positive regulation of transcription, DNA-dependent; multicellular organismal development; positive regulation of receptor internalization; positive regulation of caspase activity; positive regulation of collateral sprouting in the absence of injury; palate development; Wnt receptor signaling pathway through beta-catenin; regulation of axonogenesis; signal transduction; negative regulation of axon extension involved in axon guidance; negative regulation of neurogenesis; Wnt receptor signaling pathway in forebrain neuroblast division; anterior/posterior pattern formation; BMP signaling pathway; mammary gland development; cell-cell signaling; regulation of cell differentiation; positive regulation of cell proliferation; positive regulation of B cell proliferation; somatic stem cell division; mesoderm development; hemopoiesis; heart looping; dorsoventral neural tube patterning; positive regulation of cytokine production; platelet activation; inner ear morphogenesis; somitogenesis; Wnt receptor signaling pathway; in utero embryonic development; hippocampus development; negative regulation of fat cell differentiation; positive regulation of peptidyl-serine phosphorylation; osteoblast differentiation; paraxial mesodermal cell fate commitment; organ morphogenesis; axonogenesis; signalosome assembly; spinal cord association neuron differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; determination of left/right symmetry