WBSCR22 sirna

WBSCR22 siRNA (Mouse)

Cat. Num. AAA8215087

• Gene Names: Wbscr22; 1110003N24Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: WBSCR22; WBSCR22 siRNA (Mouse); Ribosome biogenesis methyltransferase WBSCR22; Williams-Beuren syndrome chromosomal region 22 protein homolog; WBSCR22 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: WBSCR22 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 281

• Applicable Applications for WBSCR22 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse WBSCR22 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for WBSCR22 sirna

siRNA to inhibit WBSCR22 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 125988405
• NCBI GeneID: 66138
• NCBI Accession #: NP_079651.2
• NCBI GenBank Nucleotide #: NM_025375.3
• UniProt Accession #: Q9CY21; Q3U915
• Molecular Weight: 31,587 Da
• NCBI Official Full Name: probable 18S rRNA (guanine-N(7))-methyltransferase
• NCBI Official Synonym Full Names: Williams Beuren syndrome chromosome region 22
• NCBI Official Symbol: Wbscr22
• NCBI Official Synonym Symbols: 1110003N24Rik
• NCBI Protein Information: probable 18S rRNA (guanine-N(7))-methyltransferase
• UniProt Protein Name: Probable 18S rRNA (guanine-N(7))-methyltransferase
• Protein Family: Probable 18S rRNA
• UniProt Gene Name: Wbscr22
• UniProt Entry Name: WBS22_MOUSE

Uniprot Description

WBSCR22: Methyltransferase that may act on DNA. WBSCR22 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of WBSCR22 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease. Belongs to the methyltransferase superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - histidine; Other Amino Acids Metabolism - selenoamino acid; Methyltransferase; Lipid Metabolism - androgen and estrogen; EC 2.1.1.-; Amino Acid Metabolism - tyrosine

Cellular Component: cytoplasm; nucleus

Molecular Function: methyltransferase activity; monomethylamine methyltransferase activity; trimethylamine methyltransferase activity; RNA methyltransferase activity; tRNA (cytosine)-methyltransferase activity; rRNA (cytosine-C5-967)-methyltransferase activity; rRNA (uridine) methyltransferase activity; N-methyltransferase activity; tRNA (guanine) methyltransferase activity; rRNA (adenine-N6,N6-)-dimethyltransferase activity; arginine N-methyltransferase activity; selenocysteine methyltransferase activity; protein-arginine N-methyltransferase activity; methanol-specific methylcobalamin:coenzyme M methyltransferase activity; tRNA (guanine-N2-)-methyltransferase activity; dimethylamine methyltransferase activity; protein methyltransferase activity; 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity; hydroxyneurosporene-O-methyltransferase activity; transferase activity; rRNA (uridine-2'-O-)-methyltransferase activity; tRNA (uracil) methyltransferase activity; rRNA (adenine) methyltransferase activity; C-methyltransferase activity; cobalt-precorrin-6B C5-methyltransferase activity; cobalt-precorrin-3 C17-methyltransferase activity; 1-phenanthrol methyltransferase activity; S-methyltransferase activity; lysine N-methyltransferase activity; DNA-methyltransferase activity; tRNA (cytosine-5-)-methyltransferase activity; C-terminal protein carboxyl methyltransferase activity; tRNA (adenine)-methyltransferase activity; rRNA (cytosine) methyltransferase activity; cobalt-precorrin-7 C15-methyltransferase activity; protein-arginine N5-methyltransferase activity; rRNA methyltransferase activity; protein-leucine O-methyltransferase activity; demethylmenaquinone methyltransferase activity; rRNA (adenine-N6-)-methyltransferase activity; tRNA methyltransferase activity; rRNA (guanine) methyltransferase activity; methylarsonite methyltransferase activity; mRNA methyltransferase activity; methylamine-specific methylcobalamin:coenzyme M methyltransferase activity; tRNA (guanine-N1-)-methyltransferase activity; cobalt-precorrin-5B C1-methyltransferase activity; protein-lysine N-methyltransferase activity; O-methyltransferase activity; tRNA (adenine-57, 58-N(1)-) methyltransferase activity; tRNA (adenine-N1-)-methyltransferase activity

Biological Process: methylation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; metabolic process; ribosome biogenesis and assembly; chromatin modification; rRNA processing