WASL sirna

WASL siRNA (Mouse)

Cat. Num. AAA8216196

• Gene Names: Wasl; N-WASP; 2900021I12Rik; 3110031I02Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: WASL; WASL siRNA (Mouse); Neural Wiskott-Aldrich syndrome protein; N-WASP; WASL sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: WASL siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 501

• Applicable Applications for WASL sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse WASL gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for WASL sirna

siRNA to inhibit WASL expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 268370040
• NCBI GeneID: 73178
• NCBI Accession #: NP_082735.2
• NCBI GenBank Nucleotide #: NM_028459.2
• UniProt Accession #: Q91YD9
• Molecular Weight: 54,274 Da
• NCBI Official Full Name: neural Wiskott-Aldrich syndrome protein isoform 1
• NCBI Official Synonym Full Names: Wiskott-Aldrich syndrome-like (human)
• NCBI Official Symbol: Wasl
• NCBI Official Synonym Symbols: N-WASP; 2900021I12Rik; 3110031I02Rik
• NCBI Protein Information: neural Wiskott-Aldrich syndrome protein
• UniProt Protein Name: Neural Wiskott-Aldrich syndrome protein
• Protein Family: Neural Wiskott-Aldrich syndrome protein
• UniProt Gene Name: Wasl
• UniProt Synonym Gene Names: N-WASP
• UniProt Entry Name: WASL_MOUSE

Uniprot Description

N-WASP: Regulates actin polymerization by stimulating the actin- nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. Binds actin and the Arp2/3 complex. Interacts with CDC42. Binds to SH3 domains of GRB2. Interacts with the C-terminal SH3 domain of DNMBP. Interacts with SNX9. Interacts with the WW domains of PRPF40A/FBP11. Interacts with PTK2/FAK1. Interacts with NOSTRIN. Interacts with Shigella flexneri protein IcsA. The interaction with IcsA enhances the affinity of WASL for Arp2/3, thus assembling a tight complex which has maximal activity in actin assembly. Interacts with E.coli effector protein EspF(U). Identified in a complex containing at least WASL, BAIAP2L1 and E.coli EspF(U). Binds to TNK2.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Cellular Component: Golgi membrane; cytoskeleton; lamellipodium; cytoplasm; cytoplasmic membrane-bound vesicle; nucleus; actin cap

Molecular Function: identical protein binding; protein binding; actin binding

Biological Process: positive regulation of filopodium formation; mitosis; transcription, DNA-dependent; vesicle organization and biogenesis; actin filament organization; cell projection biogenesis; cell cycle; spindle localization; actin nucleation; vesicle transport along actin filament; regulation of protein localization; response to bacterium; regulation of transcription, DNA-dependent; cell division; membrane invagination; actin cytoskeleton organization and biogenesis; membrane budding