VPS4B sirna

VPS4B siRNA (Mouse)

Cat. Num. AAA8211268

• Gene Names: Vps4b; Skd1; 8030489C12Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: VPS4B; VPS4B siRNA (Mouse); SKD1; Vacuolar protein sorting-associated protein 4B; Suppressor of K(+) transport growth defect 1; Protein SKD1; VPS4B sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: VPS4B siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 444

• Applicable Applications for VPS4B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse VPS4B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for VPS4B sirna

siRNA to inhibit VPS4B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 164698506
• NCBI GeneID: 20479
• NCBI Accession #: NP_033216.2
• NCBI GenBank Nucleotide #: NM_009190.2
• UniProt Accession #: P46467; Q91W22; Q9R1C9
• Molecular Weight: 49,419 Da
• NCBI Official Full Name: vacuolar protein sorting-associated protein 4B
• NCBI Official Synonym Full Names: vacuolar protein sorting 4b (yeast)
• NCBI Official Symbol: Vps4b
• NCBI Official Synonym Symbols: Skd1; 8030489C12Rik
• NCBI Protein Information: vacuolar protein sorting-associated protein 4B
• UniProt Protein Name: Vacuolar protein sorting-associated protein 4B
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: Vps4b
• UniProt Synonym Gene Names: Skd1; Protein SKD1
• UniProt Entry Name: VPS4B_MOUSE

Uniprot Description

VPS4B: Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Belongs to the AAA ATPase family.

Protein type: EC 3.6.4.6; Vesicle

Cellular Component: spindle pole; centrosome; membrane; lysosome; cytoplasm; vacuolar membrane; early endosome; late endosome; endosome membrane; cytosol; nucleus; endosome

Molecular Function: protein C-terminus binding; protein binding; hydrolase activity; ATPase activity; nucleotide binding; ATPase activity, coupled; ATP binding

Biological Process: cholesterol transport; endosome organization and biogenesis; cell separation during cytokinesis; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; regulation of viral reproduction; intracellular cholesterol transport; response to lipid; endosome transport; cell cycle; protein depolymerization; endosome to lysosome transport via multivesicular body sorting pathway; mitotic metaphase plate congression; protein transport; transport; cell division; nuclear organization and biogenesis; potassium ion transport; vacuole organization and biogenesis